Rabbit PARN Polyclonal Antibody | anti-PARN antibody

PARN Antibody

Cat. Num. AAA9612382

• Gene Names: PARN; DAN; DKCB6; PFBMFT4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PARN; Polyclonal Antibody; PARN Antibody; DAN; Deadenylating nuclease; Deadenylation nuclease; PARN_HUMAN; Poly A specific ribonuclease; Poly(A) specific ribonuclease; Poly(A)-specific ribonuclease PARN; Polyadenylate specific ribonuclease; Polyadenylate-specific ribonuclease; anti-PARN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PARN Antibody detects endogenous levels of total PARN.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 639

• Applicable Applications for anti-PARN antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PARN, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, using PARN Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-PARN antibody

Function: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (PubMed:10882133, PubMed:11359775, PubMed:12748283, PubMed:15175153, PubMed:9736620). Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability (PubMed:25049417, PubMed:22442037).

Post Translational Modifications: Phosphorylation by MAPKAPK2, preventing GADD45A mRNA degradation after genotoxic stress.

Subcellular Location: Nucleus. Cytoplasm. Nucleus>Nucleolus. Note: Some nuclear fraction is nucleolar.

Tissue Specificity: Ubiquitous.

Subunit Structure: Homodimer (PubMed:10801819, PubMed:16281054). Found in a mRNA decay complex with RENT1, RENT2 and RENT3B (PubMed:14527413). Interacts with KHSRP (PubMed:15175153). Interacts with CELF1/CUGBP1 (PubMed:16601207). Interacts with ZC3HAV1 in an RNA-independent manner (PubMed:21876179). Interacts with DHX36 (PubMed:14731398).

Similarity: Belongs to the CAF1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 60390262
• NCBI GeneID: 5073
• UniProt Accession #: O95453
• Molecular Weight: Observed Molecular Weight: (Observed)73kD.; Predicted Molecular Weight: (Calculated)73kDa.
• NCBI Official Full Name: Poly(A)-specific ribonuclease PARN
• NCBI Official Synonym Full Names: poly(A)-specific ribonuclease
• NCBI Official Symbol: PARN
• NCBI Official Synonym Symbols: DAN; DKCB6; PFBMFT4
• NCBI Protein Information: poly(A)-specific ribonuclease PARN
• UniProt Protein Name: Poly(A)-specific ribonuclease PARN
• Protein Family: Poly(A)-specific ribonuclease
• UniProt Gene Name: PARN
• UniProt Synonym Gene Names: DAN
• UniProt Entry Name: PARN_HUMAN

NCBI Description

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

PARN: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Ubiquitous. Belongs to the CAF1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ribonuclease; RNA-binding; EC 3.1.13.4; Nucleolus

Chromosomal Location of Human Ortholog: 16p13

Cellular Component: cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; poly(A)-specific ribonuclease activity; nuclease activity; mRNA 3'-UTR binding; metal ion binding; nucleotide binding; protein kinase binding

Biological Process: poly(A) tail shortening; RNA modification; mRNA catabolic process, nonsense-mediated decay; female gamete generation; gene expression; mRNA catabolic process, deadenylation-dependent decay

Disease: Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 4; Dyskeratosis Congenita, Autosomal Recessive, 6