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Immunoprecipitation (IP) (WB and IP: Samples: A. Whole cell lysate (100 mcg/lane) from MCF7 cells that were either untreated (-) or treated with etoposide (+; 20 micromolar, 5 h). B. Whole cell lysate (2 mg) from 293T cells. MBS617015 was used at 0.04ug/ml for WB (A) or 2.5ug/mg lysate for IP. (B). Immunoprecipitated HdmX/MDM4 was detected using MBS617015. Detection: Chemiluminescence.)

Rabbit anti-Human HdmX Polyclonal Antibody

HdmX (MDM4, HdmX, MdmX -Hdm4, Homolog of Mouse Double Minute 4 Protein)

Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
HdmX; Polyclonal Antibody; HdmX (MDM4; MdmX -Hdm4; Homolog of Mouse Double Minute 4 Protein); Anti -HdmX (MDM4; anti-HdmX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HdmX/MDM4 protein. Minimal sequence homology with mouse MdmX.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
Applicable Applications for anti-HdmX antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation.
Dilution: Immunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:10,000-1:25,000
Immunogen
A synthetic peptide which maps to a region between aa125-175 of human ortholog of mouse double minute 4 (Swiss-Prot O15151).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunoprecipitation (IP)

(WB and IP: Samples: A. Whole cell lysate (100 mcg/lane) from MCF7 cells that were either untreated (-) or treated with etoposide (+; 20 micromolar, 5 h). B. Whole cell lysate (2 mg) from 293T cells. MBS617015 was used at 0.04ug/ml for WB (A) or 2.5ug/mg lysate for IP. (B). Immunoprecipitated HdmX/MDM4 was detected using MBS617015. Detection: Chemiluminescence.)

Immunoprecipitation (IP) (WB and IP: Samples: A. Whole cell lysate (100 mcg/lane) from MCF7 cells that were either untreated (-) or treated with etoposide (+; 20 micromolar, 5 h). B. Whole cell lysate (2 mg) from 293T cells. MBS617015 was used at 0.04ug/ml for WB (A) or 2.5ug/mg lysate for IP. (B). Immunoprecipitated HdmX/MDM4 was detected using MBS617015. Detection: Chemiluminescence.)
Related Product Information for anti-HdmX antibody
HdmX/MDM-4 is the human homolog of the murine double minute 2 (Mdm2) protein. It is a critical negative regulator of the p53 tumor suppressor.
Product Categories/Family for anti-HdmX antibody

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Product Notes

The HdmX (Catalog #AAA617015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HdmX (MDM4, HdmX, MdmX -Hdm4, Homolog of Mouse Double Minute 4 Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HdmX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Dilution: Immunoprecipitation: 2-5ug/mg lysate Western Blot: 1:10,000-1:25,000. Researchers should empirically determine the suitability of the HdmX for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HdmX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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