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Western Blot (WB) (Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PTHR2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit Parathyroid Hormone Receptor 2 Polyclonal Antibody | anti-PTHR2 antibody

Parathyroid Hormone Receptor 2 (PTHR2) Polyclonal Antibody

Gene Names
Pth2r; Pthr2
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Parathyroid Hormone Receptor 2; Polyclonal Antibody; Parathyroid Hormone Receptor 2 (PTHR2) Polyclonal Antibody; PTH2; PTH2R; Parathyroid Hormone 2 Receptor; anti-PTHR2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PTHR2. It has been selected for its ability to recognize PTHR2 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
0.41mg/ml (varies by lot)
Applicable Applications for anti-PTHR2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Rat
Organism Species
Rat
Fragment
PTHR2 (Asp27~Tyr143)
Quality Control
Content: The quality control contains recombinant PTHR2 (Asp27~Tyr143) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042798
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PTHR2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PTHR2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Kidney Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,804 Da
NCBI Official Full Name
parathyroid hormone 2 receptor
NCBI Official Synonym Full Names
parathyroid hormone 2 receptor
NCBI Official Symbol
Pth2r
NCBI Official Synonym Symbols
Pthr2
NCBI Protein Information
parathyroid hormone 2 receptor
UniProt Protein Name
Parathyroid hormone 2 receptor
UniProt Gene Name
Pth2r
UniProt Synonym Gene Names
Pthr2; PTH2 receptor

NCBI Description

binds parathyroid hormone and induces cAMP accumulation [RGD, Feb 2006]

Uniprot Description

PTH2R: This is a specific receptor for parathyroid hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. PTH2R may be responsible for PTH effects in a number of physiological systems. It may play a significant role in pancreatic function. PTH2R presence in neurons indicates that it may function as a neurotransmitter receptor. Belongs to the G-protein coupled receptor 2 family.

Protein type: GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; parathyroid hormone receptor activity

Biological Process: cell surface receptor signaling pathway; G-protein coupled receptor signaling pathway

Research Articles on PTHR2

Similar Products

Product Notes

The PTHR2 pth2r (Catalog #AAA2032470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Parathyroid Hormone Receptor 2 (PTHR2) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Parathyroid Hormone Receptor 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PTHR2 pth2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parathyroid Hormone Receptor 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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