Rabbit Parathyroid Hormone (PTH) Polyclonal Antibody | anti-PTH antibody

Parathyroid Hormone (PTH) (N-Terminal)

Cat. Num. AAA4381207

• Gene Names: PTH; PTH1
• Reactivity: Human; Predicted: Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer, Orangutan
• Applications: Immunohistochemistry
• Synonyms: Parathyroid Hormone (PTH); Polyclonal Antibody; Parathyroid Hormone (PTH) (N-Terminal); hPTH; Parathormone; Parathyrin; Parathyroid hormone 1 (PTH1); Parathyroid hormone (PTH); anti-PTH antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer, Orangutan
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Epitope of this PAb maps in the N-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
• Form/Format: 200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 115

• Applicable Applications for anti-PTH antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Positive Control: Human parathyroid gland carcinoma.
• Immunogen: A recombinant fragment around aa 1-115 of human mature-PTH-polypeptide (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic and Secreted
• Hu-chromosome Location: 11p15.3-p15.1
• Preparation and Storage: Antibody with azide: store at 2°C to 8°C.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Parathyroid Gland stained with PTH Rabbit Polyclonal Antibody.)

References

Watson, P.H. and Hanley, D.A. 1993. Parathyroid hormone: regulation of synthesis and secretion. Clin. Invest. Med. 16: 58-77.

NCBI and Uniprot Product Information

• NCBI GI #: 4506267
• NCBI GeneID: 5741
• NCBI Accession #: NP_000306.1
• NCBI GenBank Nucleotide #: NP_000306.1
• UniProt Accession #: P01270
• Molecular Weight: 9kDa
• NCBI Official Full Name: parathyroid hormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: parathyroid hormone
• NCBI Official Symbol: PTH
• NCBI Official Synonym Symbols: PTH1
• NCBI Protein Information: parathyroid hormone
• UniProt Protein Name: Parathyroid hormone
• Protein Family: Parathyroid hormone
• UniProt Gene Name: PTH
• UniProt Synonym Gene Names: PTH
• UniProt Entry Name: PTHY_HUMAN

NCBI Description

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PTH: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2- deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Belongs to the parathyroid hormone family.

Protein type: Hormone; Secreted, signal peptide; Apoptosis; Secreted

Chromosomal Location of Human Ortholog: 11p15.3-p15.1

Cellular Component: extracellular space; extracellular region

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; type 1 parathyroid hormone receptor binding; parathyroid hormone receptor binding; peptide hormone receptor binding; hormone activity

Biological Process: response to drug; transcription from RNA polymerase II promoter; positive regulation of signal transduction; induction of apoptosis by hormones; positive regulation of glycogen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; Rho protein signal transduction; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of glucose import; response to cadmium ion; response to vitamin D; response to ethanol; cAMP metabolic process; cell-cell signaling; regulation of gene expression; response to lead ion; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; skeletal development; bone resorption

Disease: Hypoparathyroidism, Familial Isolated