Western Blot (WB)
(Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human N-myc Downstream Regulated Gene 1 Protein Polyclonal Antibody | anti-NDRG1 antibody
N-myc Downstream Regulated Gene 1 Protein, NT (NDRG1, NDR1, Protein NDRG1, CAP43, Differentiation-related Gene 1 Protein, CMT4D, DRG1, DRG-1, GC4, HMSNL, Nickel-specific Induction Protein Cap43, NMSL, Reducing Agents and Tunicamycin-responsive Protein, RT
Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
N-myc Downstream Regulated Gene 1 Protein; Polyclonal Antibody; NT (NDRG1; NDR1; Protein NDRG1; CAP43; Differentiation-related Gene 1 Protein; CMT4D; DRG1; DRG-1; GC4; HMSNL; Nickel-specific Induction Protein Cap43; NMSL; Reducing Agents and Tunicamycin-responsive Protein; RT; N-myc Downstream Regulated 1; anti-NDRG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NDRG1.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-NDRG1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human NDRG1 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma with NDRG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma with NDRG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-NDRG1 antibody
References
1. Sugiyama, N., et.al., Mol. Cell Proteomics 6(6), 1103-1109 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,650 Da
NCBI Official Full Name
protein NDRG1 isoform 1
NCBI Official Synonym Full Names
N-myc downstream regulated 1
NCBI Official Symbol
NDRG1
NCBI Official Synonym Symbols
GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
NCBI Protein Information
protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein
UniProt Protein Name
Protein NDRG1
Protein Family
UniProt Gene Name
NDRG1
UniProt Synonym Gene Names
CAP43; DRG1; RTP; DRG-1; RTP
UniProt Entry Name
NDRG1_HUMAN
NCBI Description
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Research Articles on NDRG1
Similar Products
Product Notes
The NDRG1 ndrg1 (Catalog #AAA6490213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N-myc Downstream Regulated Gene 1 Protein, NT (NDRG1, NDR1, Protein NDRG1, CAP43, Differentiation-related Gene 1 Protein, CMT4D, DRG1, DRG-1, GC4, HMSNL, Nickel-specific Induction Protein Cap43, NMSL, Reducing Agents and Tunicamycin-responsive Protein, RT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's N-myc Downstream Regulated Gene 1 Protein can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NDRG1 ndrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-myc Downstream Regulated Gene 1 Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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