Rabbit PAR-1 / MARK2 Polyclonal Antibody | anti-PAR-1 antibody

Rabbit Polyclonal (IgG) to Human PAR-1 / MARK2

Cat. Num. AAA243933

• Gene Names: MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PAR-1 / MARK2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PAR-1 / MARK2; Anti-PAR-1 / MARK2 Antibody (aa562-611) IHC-plus; MARK2; ELKL motif kinase 1; EMK-1; ELKL motif kinase; EMK1; PAR-1; PAR1 homolog; Par1b; Par-1b; Ser/Thr protein kinase PAR-1B; Emk; PAR1 homolog b; Human PAR-1; anti-PAR-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MARK2 Antibody detects endogenous levels of total MARK2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 745

• Applicable Applications for anti-PAR-1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:5000), IF (1:100 - 1:500), IHC-P (10 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MARK2 (aa562-611).
• Immunogen: PAR-1 / MARK2 antibody was raised against synthetic peptide from human MARK2 (aa562-611).
• Antigen Modification: aa562-611
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-MARK2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HUVEC cells, using MARK2 antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-PAR-1 antibody

MARK2 (EMK1), a MARK type protein kinase, has been implicated in regulating polarity, cell cycle progression, and microtubule dynamics. Specifically, it is reported to phosphorylate a MAP4-related protein in vitro. EMK(-/-) mice display growth retardation and immune cell dysfunction, and disease development in these animals has suggested that loss of EMK may contribute to autoimmune disease in mammals.

Product Categories/Family for anti-PAR-1 antibody

Family: Protein Kinase; Subfamily: MARK

NCBI and Uniprot Product Information

• NCBI GI #: 86990441
• NCBI GeneID: 2011
• NCBI Accession #: NP_059672.2
• NCBI GenBank Nucleotide #: NM_017490.3
• UniProt Accession #: Q7KZI7; Q15449; Q15524; Q5XGA3; Q68A18; Q96HB3; Q96RG0
• Molecular Weight: 81,209 Da
• NCBI Official Full Name: serine/threonine-protein kinase MARK2 isoform a
• NCBI Official Synonym Full Names: MAP/microtubule affinity-regulating kinase 2
• NCBI Official Symbol: MARK2
• NCBI Official Synonym Symbols: EMK1; EMK-1; PAR-1; Par1b; Par-1b
• NCBI Protein Information: serine/threonine-protein kinase MARK2; PAR1 homolog b; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK
• UniProt Protein Name: Serine/threonine-protein kinase MARK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: MARK2
• UniProt Synonym Gene Names: EMK-1; Par-1b; Par1b
• UniProt Entry Name: MARK2_HUMAN

NCBI Description

This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]

Uniprot Description

MARK2: a CAMK protein kinase of the CAMKL group. Plays a role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. 12 isoforms of the human protein are produced by alternative promoter.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: mitochondrion; membrane; plasma membrane; actin filament; nucleus; basal cortex; lateral plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; magnesium ion binding; lipid binding; protein kinase activator activity; ATP binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; Wnt receptor signaling pathway; establishment of cell polarity; protein amino acid autophosphorylation; neuron migration; peptidyl-threonine phosphorylation; activation of protein kinase activity; mitochondrion degradation; mitochondrion localization; regulation of axonogenesis; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis