Typical Testing Data/Standard Curve (for reference only)
Monkey Soluble Interleukin 2a receptor ELISA Kit | IL-2sRa elisa kit
Monkey Soluble Interleukin 2a receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: sIL-2alphaR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sIL-2alphaR antibody and an sIL-2alphaR-HRP conjugate. The assay sample and buffer are incubated together with sIL-2alphaR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sIL-2alphaR concentration since sIL-2alphaR from samples and sIL-2alphaR-HRP conjugate compete for the anti-sIL-2alphaR antibody binding site. Since the number of sites is limited, as more sites are occupied by sIL-2alphaR from the sample, fewer sites are left to bind sIL-2alphaR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIL-2alphaR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Uniprot Description
IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10p15-p14
Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding
Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; apoptosis; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; positive regulation of T cell differentiation; negative regulation of inflammatory response; immune response; inflammatory response; activated T cell apoptosis
Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10
Research Articles on IL-2sRa
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Product Notes
The Monkey IL-2sRa il2ra (Catalog #AAA740876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740876 ELISA Kit recognizes Monkey IL-2sRa. It is sometimes possible for the material contained within the vial of "Soluble Interleukin 2a receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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