Rabbit anti-Human PAPOLG Polyclonal Antibody | anti-PAPOLG antibody

PAPOLG Rabbit Polyclonal

Cat. Num. AAA7602374

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PAPOLG; Polyclonal Antibody; PAPOLG Rabbit Polyclonal; anti-PAPOLG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 496

• Applicable Applications for anti-PAPOLG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Poly(A) polymerase gamma
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue slide using MBS7602374 (PAPOLG Antibody) at dilution of 1:50)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7602374 (PAPOLG Antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 116283528
• NCBI GeneID: 64895
• Molecular Weight: 80,127 Da
• NCBI Official Full Name: PAPOLG protein, partial
• NCBI Official Synonym Full Names: poly(A) polymerase gamma
• NCBI Official Symbol: PAPOLG
• NCBI Protein Information: poly(A) polymerase gamma
• UniProt Protein Name: Poly(A) polymerase gamma
• Protein Family: Poly(A) polymerase
• UniProt Gene Name: PAPOLG
• UniProt Synonym Gene Names: PAP2; PAPG; PAP-gamma; Neo-PAP; SRP RNA-adenylating enzyme
• UniProt Entry Name: PAPOG_HUMAN

NCBI Description

This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

PAP-gamma: a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. Shares 60% identity with poly(A) polymerase II (PAPII) at the amino acid level. Exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. Responsible for the post-transcriptional adenylation of the 3'-terminal several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA. Ten alternatively spliced isoforms have been described.

Protein type: EC 2.7.7.19; Transferase

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function: ATP binding; metal ion binding; polynucleotide adenylyltransferase activity; RNA binding

Biological Process: mRNA polyadenylation; RNA polyadenylation