Western Blot (WB)
((0.2ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PAPD5 Polyclonal Antibody | anti-PAPD5 antibody
Goat anti-PAPD5 (aa497-510) Antibody
Gene Names
PAPD5; TRF4-2
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PAPD5; Polyclonal Antibody; Goat anti-PAPD5 (aa497-510) Antibody; PAP associated domain containing 5; FLJ40270; TRF4-2; PAP-associated domain-containing protein 5; TUTase 3; terminal uridylyltransferase 3; topoisomerase-related function protein 4-2; PAP associated domain containing 5 antibody; anti-PAPD5 antibody
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TDEVATYRDWISKQ
Sequence Length
698
Applicable Applications for anti-PAPD5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 70kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 69.4kDa according to Mouse NP_001157971.1. Recommended concentration: 0.2-0.6ug/ml.
Western Blot: Approx 70kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 69.4kDa according to Mouse NP_001157971.1. Recommended concentration: 0.2-0.6ug/ml.
Immunogen
Peptide with sequence C-TDEVATYRDWISKQ, from the internal region of the protein sequence according to NP_001035374.2; NP_001035375.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_001035374.2; NP_001035375.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.2ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.2ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
non-canonical poly(A) RNA polymerase PAPD5 isoform a
NCBI Official Synonym Full Names
PAP associated domain containing 5
NCBI Official Symbol
PAPD5
NCBI Official Synonym Symbols
TRF4-2
NCBI Protein Information
non-canonical poly(A) RNA polymerase PAPD5
UniProt Protein Name
Non-canonical poly(A) RNA polymerase PAPD5
Protein Family
UniProt Gene Name
PAPD5
UniProt Synonym Gene Names
TUTase 3; TRF4-2
UniProt Entry Name
PAPD5_HUMAN
Uniprot Description
PAPD5: Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Belongs to the DNA polymerase type-B-like family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.7.19; Nucleolus; Transferase
Chromosomal Location of Human Ortholog: 16q12.1
Cellular Component: cytoplasm; nucleolus
Molecular Function: DNA binding; DNA-directed DNA polymerase activity; metal ion binding; polynucleotide adenylyltransferase activity
Biological Process: cell division; mitosis; mRNA processing; rRNA processing
Research Articles on PAPD5
Similar Products
Product Notes
The PAPD5 papd5 (Catalog #AAA422687) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PAPD5 (aa497-510) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PAPD5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 70kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 69.4kDa according to Mouse NP_001157971.1. Recommended concentration: 0.2-0.6ug/ml. Researchers should empirically determine the suitability of the PAPD5 papd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TDEVATYRDW ISKQ. It is sometimes possible for the material contained within the vial of "PAPD5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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