Western Blot (WB)
(Biotinylated (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat pan ADH Polyclonal Antibody | anti-ADH1A, B, C antibody
Goat Anti-pan ADH, Biotinylated Antibody
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat
Peptide ELISA: Antibody detection limit dilution 1:8000.
Western Blot (WB)
(Biotinylated (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]
Uniprot Description
ADH1A: a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]
Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Lipid Metabolism - fatty acid; Transcription regulation
Chromosomal Location of Human Ortholog: 4q23
Cellular Component: cytosol
Molecular Function: alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; protein binding; retinol dehydrogenase activity
Biological Process: drug metabolic process; ethanol oxidation
Research Articles on ADH1A, B, C
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Product Notes
The ADH1A, B, C adh1a (Catalog #AAA423668) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-pan ADH, Biotinylated Antibody reacts with Human, Mouse, Rat Tested: Human Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's pan ADH can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Western Blot: Approx 39kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml. Peptide ELISA: Antibody detection limit dilution 1:8000. Researchers should empirically determine the suitability of the ADH1A, B, C adh1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: STAGKVMKCK A. It is sometimes possible for the material contained within the vial of "pan ADH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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