Human Alcohol dehydrogenase 1A ELISA Kit | ADH1A elisa kit

Human Alcohol dehydrogenase 1A ELISA Kit

Cat. Num. AAA760363

• Gene Names: ADH1A; ADH1
• Reactivity: Human
• Synonyms: Alcohol dehydrogenase 1A; Human Alcohol dehydrogenase 1A ELISA Kit; ADH1 (Alcohol Dehydrogenase 1)/ADH1A/Alcohol dehydrogenase subunit alpha; ADH1A elisa kit

• Reactivity: Human
• Sequence Length: 375

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADH1A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ALCAM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ALCAM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ALCAM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ALCAM can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4501929
• NCBI GeneID: 124
• NCBI Accession #: NP_000658.1
• NCBI GenBank Nucleotide #: NM_000667.3
• Molecular Weight: 39,859 Da
• NCBI Official Full Name: alcohol dehydrogenase 1A
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1A (class I), alpha polypeptide
• NCBI Official Symbol: ADH1A
• NCBI Official Synonym Symbols: ADH1
• NCBI Protein Information: alcohol dehydrogenase 1A
• UniProt Protein Name: Alcohol dehydrogenase 1A
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH1A
• UniProt Synonym Gene Names: ADH1
• UniProt Entry Name: ADH1A_HUMAN

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Uniprot Description

ADH1A: a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Protein type: Transcription regulation; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; protein binding; zinc ion binding

Biological Process: alcohol metabolic process; drug metabolic process; ethanol oxidation; xenobiotic metabolic process