Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Rabbit anti-Human, mouse Pak4 Polyclonal Antibody | anti-Pak4 antibody
Mouse Pak4 Antibody (Center)
Gene Names
Pak4; AW555722; mKIAA1142; 5730488L07Rik
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Pak4; Polyclonal Antibody; Mouse Pak4 Antibody (Center); Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; Kiaa1142; anti-Pak4 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of mouse Pak4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
187-216
Sequence Length
593
Applicable Applications for anti-Pak4 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Flow Cytometry (FC/FACS)
(Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Pak4 antibody
Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity).
Product Categories/Family for anti-Pak4 antibody
References
Liu, Y., et al. Oncogene 29(44):5883-5894(2010)
Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009) :
Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008)
Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008)
Danzer, K.M., et al. J. Neurochem. 103(6):2401-2407(2007)
Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009) :
Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008)
Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008)
Danzer, K.M., et al. J. Neurochem. 103(6):2401-2407(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64623
NCBI Official Full Name
serine/threonine-protein kinase PAK 4
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 4
NCBI Official Symbol
Pak4
NCBI Official Synonym Symbols
AW555722; mKIAA1142; 5730488L07Rik
NCBI Protein Information
serine/threonine-protein kinase PAK 4
UniProt Protein Name
Serine/threonine-protein kinase PAK 4
Protein Family
UniProt Gene Name
Pak4
UniProt Synonym Gene Names
Kiaa1142; PAK-4
UniProt Entry Name
PAK4_MOUSE
Uniprot Description
PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily
Cellular Component: focal adhesion; cytoplasm
Molecular Function: receptor signaling protein serine/threonine kinase activity
Biological Process: response to stress; protein amino acid phosphorylation
Research Articles on Pak4
Similar Products
Product Notes
The Pak4 pak4 (Catalog #AAA9214670) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 187-216. The Mouse Pak4 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pak4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the Pak4 pak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pak4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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