Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-37 (RAB37) Standard Curve.)
Human Ras-Related Protein Rab-37 (RAB37) ELISA Kit | RAB37 elisa kit
Human Ras-Related Protein Rab-37 (RAB37) ELISA Kit
Reactivity
Human
Synonyms
Ras-Related Protein Rab-37 (RAB37); Human Ras-Related Protein Rab-37 (RAB37) ELISA Kit; RAB37; FLJ30284; FLJ32507; member of RAS oncogene family; Rab37-like; RAB37 elisa kit
Reactivity
Human
Specificity
Human Ras-related protein Rab-37 (RAB37) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB37. No significant cross-reactivity or interference between Human RAB37 and analogues was observed.
Sequence Length
223
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-37 (RAB37) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-37 (RAB37) Standard Curve.)
Related Product Information for RAB37 elisa kit
Description: This Human Ras-related protein Rab-37 (RAB37) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB37 in samples. An antibody specific for RAB37 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAB37 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB37 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB37 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al. (2000) identified Rab37 from a mouse mast cell line. The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases. It shares 74% and 47% amino acid sequence identity with rat RAB26 and mouse Rab8 (RAB8A). Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line. Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34-kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells. Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules.
Background: Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al. (2000) identified Rab37 from a mouse mast cell line. The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases. It shares 74% and 47% amino acid sequence identity with rat RAB26 and mouse Rab8 (RAB8A). Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line. Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34-kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells. Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-related protein Rab-37 isoform 2
NCBI Official Synonym Full Names
RAB37, member RAS oncogene family
NCBI Official Symbol
RAB37
NCBI Protein Information
ras-related protein Rab-37
UniProt Protein Name
Ras-related protein Rab-37
Protein Family
UniProt Gene Name
RAB37
UniProt Entry Name
RAB37_HUMAN
NCBI Description
Rab proteins are low molecular mass GTPases that are critical regulators of vesicle trafficking. For additional background information on Rab proteins, see MIM 179508.[supplied by OMIM, Apr 2006]
Uniprot Description
RAB37: Belongs to the small GTPase superfamily. Rab family. 4 isoforms of the human protein are produced by alternative splicing
Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: secretory granule membrane; plasma membrane; ER-Golgi intermediate compartment; endosome
Molecular Function: GTPase activity; GDP binding; GTP binding
Biological Process: regulation of exocytosis; intracellular protein transport; metabolic process; protein secretion; Rab protein signal transduction; vesicle docking during exocytosis
Research Articles on RAB37
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Product Notes
The Human RAB37 rab37 (Catalog #AAA9714657) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714657 ELISA Kit recognizes Human RAB37. It is sometimes possible for the material contained within the vial of "Ras-Related Protein Rab-37 (RAB37), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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