Rabbit anti-Rat PAK1 Polyclonal Antibody | anti-PAK1 antibody

Anti-PAK1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101095

• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PAK1; Polyclonal Antibody; Anti-PAK1 Antibody; Rabbit Polyclonal; PAK1 Antibody; Rabbit PAb; Antigen Affinity Purified; p21 protein (Cdc42/Rac)-activated kinase 1; anti-PAK1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat PAK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PAK1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Rat PAK1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Rat PAK1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PAK1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:65 kDa Observed band size:72 kDa (We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of PAK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-rat PAK1 polyclonal antibody (dilution ratio: 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(PAK1 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg NIH-3T3 Whole Cell Lysate 1 uL anti-PAK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PAK1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 66 kDa Observed band size: 72 kDa)

Related Product Information for anti-PAK1 antibody

P21-activated kinase-1 (PAK1) is an enzyme associated with multiple metabolic networks and different types of cancers.PAK1 (RAC/CDC42-activated kinase 1) is the major oncogenic/ageing kinase, and its dysfunction extends the healthy lifespan of C. elegans by activating HSP16 gene. The p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) expression appears to be predictive of prognosis in various solid tumors. PAK1 expression may be a predictive marker of overall survival and disease-specific survival in patients with solid tumors.p21-Activated kinase 1 (PAK1) has attracted much attention as a potential therapeutic target due to its central role in many oncogenic signaling pathways, its frequent dysregulation in cancers and neurological disorders, and its tractability as a target for small-molecule inhibition.

Product Categories/Family for anti-PAK1 antibody

EGFR Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 8393901
• NCBI GeneID: 29431
• NCBI Accession #: NP_058894.1
• NCBI GenBank Nucleotide #: NM_017198.1
• UniProt Accession #: P35465; Q62934
• Molecular Weight: 68/70
• NCBI Official Full Name: serine/threonine-protein kinase PAK 1
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 1
• NCBI Official Symbol: Pak1
• NCBI Protein Information: serine/threonine-protein kinase PAK 1; PAK-1; p68-PAK; alpha-PAK; protein kinase MUK2; p21-activated kinase 1; p21 (CDKN1A)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Pak1
• UniProt Synonym Gene Names: PAK-1
• UniProt Entry Name: PAK1_RAT

NCBI Description

serine/threonine protein kinase; binds and forms complex specifically with activated (GTP-bound) p21, leading to inhibiting p21 GTPase activity [RGD, Feb 2006]

Uniprot Description

Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Ref.5 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.5

Cofactor: Magnesium.

Enzyme regulation: Phosphorylation of Thr-84 by OXSR1 inhibits activation

By similarity. Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, and enables activation by phosphorylation of Thr-422. Ref.5 Ref.7 Ref.11

Subunit structure: Homodimer in its autoinhibited state. Active as monomer. Component of cytoplasmic complexes, which also contains PXN, ARHGEF6 and GIT1. Interacts with NISCH. Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering. Binds to the caspase-cleaved p110 isoform ofCDC2L1 and CDC2L2, p110C, but not the full-length proteins. Interacts with ARHGEF7. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interacts with NCK1 and NCK2. Interacts with TBCB. Interacts with CRIPAK. Interacts with BRSK2

By similarity. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Interacts with SNAI1

By similarity. Ref.5 Ref.11 Ref.15

Subcellular location: Cytoplasm

By similarity. Cell junction › focal adhesion

By similarity. Cell membrane

By similarity. Note: Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation

By similarity. Ref.5 Ref.7 Ref.8

Tissue specificity: Expressed predominantly in the brain, with higher expression in neuronal groups associated with motor function, and at lower levels in the spleen. Ref.4

Developmental stage: Found in the embryonic CNS with little expression elsewhere.

Post-translational modification: Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-422 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-422 by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites

By similarity. Activated by phosphorylation at Thr-422 by BRSK2. Ref.5 Ref.11 Ref.13

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.