Immunohistochemistry (IHC)
(MBS9603670 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit PAFAH2 Polyclonal Antibody | anti-PAFAH2 antibody
PAFAH2 Antibody
Gene Names
PAFAH2; HSD-PLA2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PAFAH2; Polyclonal Antibody; PAFAH2 Antibody; EC 3.1.1.47; HSD PLA2; HSDPLA2; Platelet activating factor acetylhydrolase 2; Platelet activating factor acetylhydrolase 2 cytoplasmic; platelet-activating factor acetylhydrolase 2; 40kDa; SD PLA2; Serine dependent phospholipase A2; anti-PAFAH2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PAFAH2 antibody detects endogenous levels of PAFAH2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
392
Applicable Applications for anti-PAFAH2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human PAFAH2.
Subcellular Location
Cytoplasmic
Tissue Specificity
Broadly expressed in different tissues, but high in B-and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.
Predicted Cross Reactivity
Pig, Horse, Dog
Similarity
Pig (89%), Horse (100%), Dog (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603670 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603670 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver tissue, using PAFAH2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver tissue, using PAFAH2 antibody.)
Related Product Information for anti-PAFAH2 antibody
Function: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.
Subunit Structure: Monomer.
Similarity: Belongs to the serine esterase family.
Subunit Structure: Monomer.
Similarity: Belongs to the serine esterase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 44-46 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
platelet-activating factor acetylhydrolase 2, cytoplasmic
NCBI Official Synonym Full Names
platelet activating factor acetylhydrolase 2
NCBI Official Symbol
PAFAH2
NCBI Official Synonym Symbols
HSD-PLA2
NCBI Protein Information
platelet-activating factor acetylhydrolase 2, cytoplasmic
UniProt Protein Name
Platelet-activating factor acetylhydrolase 2, cytoplasmic
Protein Family
UniProt Gene Name
PAFAH2
UniProt Synonym Gene Names
SD-PLA2Curated; hSD-PLA21 PublicationManual assertion based on opinion iniRef.2
NCBI Description
This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008]
Uniprot Description
Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.
Research Articles on PAFAH2
Similar Products
Product Notes
The PAFAH2 pafah2 (Catalog #AAA9603670) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAFAH2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAFAH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PAFAH2 pafah2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAFAH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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