Rabbit anti-Mouse Cst3 Polyclonal Antibody | anti-Cst3 antibody

Cst3, ID (Cst3, Cystatin-C, Cystatin-3) (APC)

Cat. Num. AAA6289485

• Gene Names: Cst3; CysC
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cst3; Polyclonal Antibody; ID (Cst3; Cystatin-C; Cystatin-3) (APC); Cystatin-3; anti-Cst3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Cst3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cst3 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 38-65 amino acids selected from the Central region of mouse Cst3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Cst3 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cst3 antibody detected the Cst3 protein (arrow).)

Related Product Information for anti-Cst3 antibody

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Product Categories/Family for anti-Cst3 antibody

Antibodies; Protease Inhibitors

References

Schulte, S., et al. Am. J. Pathol. 177(1):456-463(2010). Baston-Buest, D.M., et al. Reproduction 139(4):741-748(2010). Frendeus, K.H., et al. Int. J. Biochem. Cell Biol. 41(11):2262-2269(2009). Trimarchi, J.M., et al. Dev. Dyn. 238(9):2327-2329(2009). Sun, B., et al. Neuron 60(2):247-257(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 31981822
• NCBI GeneID: 13010
• NCBI Accession #: NP_034106.2
• NCBI GenBank Nucleotide #: NM_009976.4
• UniProt Accession #: P21460; Q544Y0
• Molecular Weight: 15,531 Da
• NCBI Official Full Name: cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: Cst3
• NCBI Official Synonym Symbols: CysC
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: Cst3
• UniProt Entry Name: CYTC_MOUSE

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Cellular Component: axon; basement membrane; cell projection; cell soma; contractile fiber; cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; lysosome; multivesicular body; nuclear membrane; perinuclear region of cytoplasm; vesicle

Molecular Function: beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protease inhibitor activity

Biological Process: defense response; fibril organization and biogenesis; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell proliferation; positive regulation of DNA replication; regulation of programmed cell death; response to oxidative stress