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Western Blot (WB) (Western blot analysis of lysate from human heart tissue, using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Rabbit PACSIN1 Polyclonal Antibody | anti-PACSIN1 antibody

PACSIN1 Antibody (N-term)

Gene Names
PACSIN1; SDPI
Reactivity
Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PACSIN1; Polyclonal Antibody; PACSIN1 Antibody (N-term); Protein kinase C and casein kinase substrate in neurons protein 1; Syndapin-1; KIAA1379; anti-PACSIN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PACSIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human PACSIN1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
8-38
Sequence Length
444
Applicable Applications for anti-PACSIN1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from human heart tissue, using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB) (Western blot analysis of lysate from human heart tissue, using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB)

(Western blot analysis of lysates from Daudi cell line, human brain, human pancrease, human heart tissue (from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from Daudi cell line, human brain, human pancrease, human heart tissue (from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB)

(Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(Western blot analysis of lysates from Daudi cell line, human heart and pancreas tissue lysate(from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from Daudi cell line, human heart and pancreas tissue lysate(from left to right), using PACSIN1 Antibody (G23). MBS9208200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(Western blot analysis of anti-PACSIN1 Pab in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of anti-PACSIN1 Pab in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of anti-PACSIN1 Pab in mouse brain tissue lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of anti-PACSIN1 Pab in mouse brain tissue lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-PACSIN1 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-PACSIN1 antibody
References
Sumoy, L., et al., Gene 262 (1-2), 199-205 (2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50966
NCBI Official Full Name
protein kinase C and casein kinase substrate in neurons protein 1
NCBI Official Synonym Full Names
protein kinase C and casein kinase substrate in neurons 1
NCBI Official Symbol
PACSIN1
NCBI Official Synonym Symbols
SDPI
NCBI Protein Information
protein kinase C and casein kinase substrate in neurons protein 1
UniProt Protein Name
Protein kinase C and casein kinase substrate in neurons protein 1
UniProt Gene Name
PACSIN1
UniProt Synonym Gene Names
KIAA1379
UniProt Entry Name
PACN1_HUMAN

Uniprot Description

PACSIN1: May play a role in vesicle formation and transport. Belongs to the PACSIN family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: COPI-coated vesicle; neuron projection; cytoplasmic vesicle membrane; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; nerve terminal; cell junction; cytosol; endosome

Molecular Function: protein binding; cytoskeletal protein binding; phospholipid binding

Biological Process: negative regulation of endocytosis; regulation of endocytosis; synaptic vesicle endocytosis; actin filament organization; cytoskeleton organization and biogenesis; neurite morphogenesis

Research Articles on PACSIN1

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Product Notes

The PACSIN1 pacsin1 (Catalog #AAA9208200) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 8-38. The PACSIN1 Antibody (N-term) reacts with Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PACSIN1 pacsin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACSIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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