Rabbit PABP1 Polyclonal Antibody | anti-PABP1 antibody

Phospho-PABP1 (Tyr364) Antibody

Cat. Num. AAA9613140

• Gene Names: PABPC1; PAB1; PABP; PABP1; PABPC2; PABPL1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PABP1; Polyclonal Antibody; Phospho-PABP1 (Tyr364) Antibody; PAB 1; PAB1; PABP 1; PABPC 1; PABPC1; PABPC2; PABPL1; Poly A binding protein 1; Poly A binding protein cytoplasmic 1; poly (A) binding protein; cytoplasmic 1; cytoplasmic 2; Polyadenylate binding protein 1; anti-PABP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PABP1 (Tyr364) Antibody detects endogenous levels of PABP1 only when phosphorylated at Tyr364.; Tissue Specificity: Ubiquitous.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PABP1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PABP1 around the phosphorylation site of Tyr364.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by MAPKAPK2.Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.
• Subunit Structure: May interact with SETX. May form homodimers. Component of a multisubunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Directly interacts with IGF2BP1. Part of a complex associated with the FOS mCRD domain and consisting of HNRPD, SYNCRIP, PAIP1 and CSDE1/UNR. Interacts with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1 and PAIP2. Interacts with PAIP1 with a 1:1 stoichiometry and with PAIP2 with a 1:2 stoichiometry. The interaction with CSDE1 is direct and RNA-independent. Found in a mRNP complex with YBX2 (By similarity). Interacts with TENT2/GLD2 (By similarity). Identified in the spliceosome C complex. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with NFX1. Interacts with PIWIL1 (By similarity). Interacts with AGO1, AGO2, GSPT1 and GSPT2 Ref.53). Interacts with LARP4B (Ref.55). Interacts (via the second and third RRM domains and the C-terminus) with PAIP2B (via central acidic portion and C-terminus). Interacts with LARP1. Interacts with SHFL. Found in a complex with SHFL and LARP1. Interacts with LARP4. Interacts with ZFC3H1 in a RNase-sensitive manner. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner. Interacts with TENT5C; the interaction has no effect on TENT5C poly (A) polymerase function. Interacts with G3BP1 and G3BP2. Interacts with DDX3X. Interacts with NXF1/TAP. (Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.
• Similarity: The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively.The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively.Belongs to the polyadenylate-binding protein type-1 family.
• Subcellular Location: Cytoplasm. Nucleus. Cytoplasm>Stress granule.; Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs, which may be different from stress granules (PubMed:17289661). Shuttles between the cytoplasm and the nucleus (PubMed:9582337).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HCT116  , using Phospho-PABP1(Tyr364) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-PABP1 antibody

Binds the poly (A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly (A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly (A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability. (Microbial infection) Positively regulates the replication of dengue virus (DENV).

NCBI and Uniprot Product Information

• NCBI GI #: 3183544
• NCBI GeneID: 26986
• UniProt Accession #: P11940; Q15097; Q93004
• Molecular Weight: 636
• NCBI Official Full Name: Polyadenylate-binding protein 1
• NCBI Official Synonym Full Names: poly(A) binding protein, cytoplasmic 1
• NCBI Official Symbol: PABPC1
• NCBI Official Synonym Symbols: PAB1; PABP; PABP1; PABPC2; PABPL1
• NCBI Protein Information: polyadenylate-binding protein 1; poly(A) binding protein, cytoplasmic 2
• UniProt Protein Name: Polyadenylate-binding protein 1
• UniProt Gene Name: PABPC1
• UniProt Synonym Gene Names: PAB1; PABP1; PABPC2; PABP-1; Poly(A)-binding protein 1
• UniProt Entry Name: PABP1_HUMAN

NCBI Description

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010]

Uniprot Description

PABP 1: poly(A) binding protein 1. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Shuttles between the cytoplasm and the nucleus. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. A potential substrate in MAPKAP kinase 2-induced mRNA stabilization. Two alternatively spliced isoforms have been described.

Protein type: Spliceosome; Translation; Motility/polarity/chemotaxis; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 8q22.2-q23

Cellular Component: focal adhesion; membrane; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; translation activator activity; nucleotide binding; poly(U) binding; poly(A) binding

Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; cellular protein metabolic process; translation; positive regulation of translation; mRNA stabilization; mRNA catabolic process, nonsense-mediated decay; translational initiation; mRNA polyadenylation; gene expression; RNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay