Rabbit anti-Human PABPC1 Polyclonal Antibody | anti-PABPC1 antibody

PABPC1 (Polyadenylate-binding Protein 1, Poly(A)-binding Protein 1, PABP 1, PABPC2, PABP1, PAB1) (FITC)

Cat. Num. AAA6388242

• Gene Names: PABPC1; PAB1; PABP; PABP1; PABPC2; PABPL1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PABPC1; Polyclonal Antibody; PABPC1 (Polyadenylate-binding Protein 1; Poly(A)-binding Protein 1; PABP 1; PABPC2; PABP1; PAB1) (FITC); anti-PABPC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PABPC1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2869

• Applicable Applications for anti-PABPC1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PABPC1, aa1-636 (NP_002559.2).
• Immunogen Sequence: MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PABPC1 expression in transfected 293T cell line by PABPC1 polyclonal antibody. Lane 1: PABPC1 transfected lysate (70.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PABPC1 antibody

PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

Product Categories/Family for anti-PABPC1 antibody

Antibodies; RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 56676313
• NCBI GeneID: 26986
• NCBI Accession #: NM_002568.3
• NCBI GenBank Nucleotide #: NM_002568.3
• UniProt Accession #: P11940
• NCBI Official Full Name: Homo sapiens poly(A) binding protein cytoplasmic 1 (PABPC1), mRNA
• NCBI Official Synonym Full Names: poly(A) binding protein cytoplasmic 1
• NCBI Official Symbol: PABPC1
• NCBI Official Synonym Symbols: PAB1; PABP; PABP1; PABPC2; PABPL1
• NCBI Protein Information: polyadenylate-binding protein 1
• UniProt Protein Name: Polyadenylate-binding protein 1
• UniProt Gene Name: PABPC1
• UniProt Synonym Gene Names: PAB1; PABP1; PABPC2; PABP-1; Poly(A)-binding protein 1
• UniProt Entry Name: PABP1_HUMAN

NCBI Description

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010]

Uniprot Description

PABP 1: poly(A) binding protein 1. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Shuttles between the cytoplasm and the nucleus. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. A potential substrate in MAPKAP kinase 2-induced mRNA stabilization. Two alternatively spliced isoforms have been described.

Protein type: Spliceosome; Translation; Motility/polarity/chemotaxis; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 8q22.2-q23

Cellular Component: focal adhesion; membrane; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; translation activator activity; nucleotide binding; poly(U) binding; poly(A) binding

Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; cellular protein metabolic process; translation; positive regulation of translation; mRNA stabilization; mRNA catabolic process, nonsense-mediated decay; translational initiation; mRNA polyadenylation; gene expression; RNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay