Rabbit anti-Human PABPC1 Polyclonal Antibody | anti-PABPC1 antibody

Anti-PABPC1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103182

• Gene Names: PABPC1; PAB1; PABP; PABP1; PABPC2; PABPL1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PABPC1; Polyclonal Antibody; Anti-PABPC1 Antibody; Rabbit Polyclonal; PABPC1 Antibody; Rabbit PAb; Antigen Affinity Purified; poly(A) binding protein; cytoplasmic 1; Anti-PAB1 Antibody; Anti-PABP Antibody; Anti-PABP1 Antibody; Anti-PABPC2 Antibody; Anti-PABPL1 Antibody; anti-PABPC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PABPC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PABPC1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PABPC1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PABPC1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PABPC1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:71 kDa Observed band size:71 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PABPC1 in human colon carcinoma with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PABPC1 in human lung cancer with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PABPC1 antibody

Polyadenylate-binding protein cytoplasmic 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation and both RNA processing and stability. PABPC1 controls the rate of mRNA deadenylation and participates in mRNA decay, which is involved in carcinogenesis. PABPC1 is an important cellular target of viral ORF57 to directly upregulate PAN accumulation during viral lytic infection, and the ability of host PABPC1 to disrupt ORF57 expression is a strategic host counterbalancing mechanism. Embryonic poly(A)-binding protein (EPAB) and poly(A)-binding protein, cytoplasmic 1 (PABPC1) bind poly(A) tails of mRNAs and mediate their translational regulation in germ cells and early preimplantation embryos. PABPC1 gene was identified as a downstream target of SNHG14, and PABPC1 mediates the SNHG14-induced oncogenic effects.

References

Ozturk S, et al. (2015) Epab and pabpc1 are differentially expressed in the postnatal mouse ovaries. J Assist Reprod Genet 32 (1): 137-146.Massimelli MJ, et al. (2013) Interplay between polyadenylate-binding protein 1 and kaposi's sarcoma-associated herpesvirus orf57 in accumulation of polyadenylated nuclear rna, a viral long noncoding rna. J Virol 87 (1): 243-256.Dong H, et al. (2018) Long non-coding rna snhg14 induces trastuzumab resistance of breast cancer via regulating pabpc1 expression through h3k27 acetylation. J Cell Mol Med 22 (10): 4935-4947.

NCBI and Uniprot Product Information

• NCBI GI #: 3183544
• NCBI GeneID: 26986
• UniProt Accession #: P11940; Q15097; Q93004
• Molecular Weight: 636
• NCBI Official Full Name: Polyadenylate-binding protein 1
• NCBI Official Synonym Full Names: poly(A) binding protein, cytoplasmic 1
• NCBI Official Symbol: PABPC1
• NCBI Official Synonym Symbols: PAB1; PABP; PABP1; PABPC2; PABPL1
• NCBI Protein Information: polyadenylate-binding protein 1; poly(A) binding protein, cytoplasmic 2
• UniProt Protein Name: Polyadenylate-binding protein 1
• UniProt Gene Name: PABPC1
• UniProt Synonym Gene Names: PAB1; PABP1; PABPC2; PABP-1; Poly(A)-binding protein 1
• UniProt Entry Name: PABP1_HUMAN

NCBI Description

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010]

Uniprot Description

PABP 1: poly(A) binding protein 1. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Shuttles between the cytoplasm and the nucleus. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. A potential substrate in MAPKAP kinase 2-induced mRNA stabilization. Two alternatively spliced isoforms have been described.

Protein type: Spliceosome; Translation; Motility/polarity/chemotaxis; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 8q22.2-q23

Cellular Component: focal adhesion; membrane; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; translation activator activity; nucleotide binding; poly(U) binding; poly(A) binding

Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; cellular protein metabolic process; translation; positive regulation of translation; mRNA stabilization; mRNA catabolic process, nonsense-mediated decay; translational initiation; mRNA polyadenylation; gene expression; RNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay