Human Deoxyribonuclease X (DNASEX) ELISA Kit | DNASEX elisa kit

Human Deoxyribonuclease X (DNASEX) ELISA Kit

Cat. Num. AAA456055

• Gene Names: DNASE1L1; XIB; G4.8; DNL1L; DNASEX; DNAS1L1
• Reactivity: Human
• Synonyms: Deoxyribonuclease X (DNASEX); Human Deoxyribonuclease X (DNASEX) ELISA Kit; DNASEX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNASEX. No significant cross-reactivity or interference between DNASEX and analogues was observed.
• Sequence Length: 302

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNASEX were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNASEX were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNASEX elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DNASEX in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNASEX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNASEX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNASEX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DNASEX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 58430942
• NCBI GeneID: 1774
• NCBI Accession #: NP_001009932.1
• NCBI GenBank Nucleotide #: NM_001009932.2
• UniProt Accession #: P49184; Q5HY41; D3DWW7
• Molecular Weight: 33,893 Da
• NCBI Official Full Name: deoxyribonuclease-1-like 1
• NCBI Official Synonym Full Names: deoxyribonuclease 1 like 1
• NCBI Official Symbol: DNASE1L1
• NCBI Official Synonym Symbols: XIB; G4.8; DNL1L; DNASEX; DNAS1L1
• NCBI Protein Information: deoxyribonuclease-1-like 1
• UniProt Protein Name: Deoxyribonuclease-1-like 1
• UniProt Gene Name: DNASE1L1
• UniProt Synonym Gene Names: DNAS1L1; DNL1L; DNase I-like 1

NCBI Description

This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed. [provided by RefSeq, Jan 2015]