Western Blot (WB)
(Western Blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control:Lane 1: SiHa Lane 2: K562Lane 3: A431)
Rabbit anti-Human p60 CAF1 Polyclonal Antibody | anti-CAF1 antibody
p60 CAF1 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
p60 CAF1; Polyclonal Antibody; p60 CAF1 Polyclonal Antibody; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF 1 subunit B; CAF 1A; CAF I 60 kDa subunit; CAF IP60; CAF-1; CAF1; CAF1B; CHAF 1B; Chromatin assembly factor 1 subunit B; Chromatin assembly factor I; Chromatin assembly factor I p60 subunit; Human chromatin assembly factor-I p60 subunit; M phase phosphoprotein 7; M-phase phosphoprotein 7; MPP 7; p60 subunit; anti-CAF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
p60 CAF1 polyclonal antibody detects endogenous levels of p60 CAF1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
559
Applicable Applications for anti-CAF1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control:Lane 1: SiHa Lane 2: K562Lane 3: A431)
Western Blot (WB)
(Western Blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control:Lane 1: SiHa Lane 2: K562Lane 3: A431)
Immunocytochemistry (ICC)
(ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-CAF1 antibody
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1alpha, HP1beta and HP1gamma). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61kDa
NCBI Official Full Name
chromatin assembly factor 1 subunit B
UniProt Protein Name
Chromatin assembly factor 1 subunit B
Protein Family
UniProt Gene Name
CHAF1B
UniProt Synonym Gene Names
CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60
UniProt Entry Name
CAF1B_HUMAN
Uniprot Description
CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; cytosol; nucleus
Molecular Function: histone binding; unfolded protein binding; chromatin binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; cell cycle; reproduction; DNA repair; DNA replication; DNA replication-dependent nucleosome assembly
Similar Products
Product Notes
The CAF1 chaf1b (Catalog #AAA3013191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p60 CAF1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p60 CAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IF: 1:50-1:100 IHC: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CAF1 chaf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p60 CAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
