Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution.)
Rabbit CAF-1 Polyclonal Antibody | anti-CAF-1 antibody
CAF-1 p60 Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:20-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody at dilution of 1:200 (40x lens).)
NCBI and Uniprot Product Information
Uniprot Description
CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; cytosol; nucleus
Molecular Function: histone binding; unfolded protein binding; chromatin binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; cell cycle; reproduction; DNA repair; DNA replication; DNA replication-dependent nucleosome assembly
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Product Notes
The CAF-1 chaf1b (Catalog #AAA3009887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAF-1 p60 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAF-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100. Researchers should empirically determine the suitability of the CAF-1 chaf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAF-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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