Western Blot (WB)
(Western blot analysis of p47 phox (pS345) expression in HepG2 (A), NIH3T3 (B) whole cell lysates.)
Rabbit anti-Human, Mouse p47 phox Polyclonal Antibody | anti-NCF1 antibody
Anti-p47 phox (pS345) Phospho Antibody
Western Blot (WB)
(Western blot analysis of p47 phox (pS345) expression in HepG2 (A), NIH3T3 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p47 phox (pS345) expression in HepG2 (A), NIH3T3 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p47 phox (pS345) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p47 phox (pS345) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Uniprot Description
p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.
Protein type: Oxidoreductase; EC 1.-.-.-
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex
Molecular Function: electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell proliferation; cell redox homeostasis; cellular defense response; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; leukotriene metabolic process; negative regulation of smooth muscle contraction; protein targeting to membrane; respiratory burst; respiratory burst during defense response; response to yeast; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway
Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I
Research Articles on NCF1
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Product Notes
The NCF1 ncf1 (Catalog #AAA8242830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p47 phox (pS345) Phospho Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p47 phox can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the NCF1 ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p47 phox, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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