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Western Blot (WB) (MRPL9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MRPL9 antibody detected the MRPL9 protein (arrow).)

Rabbit MRPL9 Polyclonal Antibody | anti-MRPL9 antibody

MRPL9 Antibody (N-term)

Gene Names
MRPL9; L9mt
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MRPL9; Polyclonal Antibody; MRPL9 Antibody (N-term); 39S ribosomal protein L9; mitochondrial; L9mt; MRP-L9; anti-MRPL9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MRPL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human MRPL9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
66-95
Sequence Length
267
Applicable Applications for anti-MRPL9 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(MRPL9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MRPL9 antibody detected the MRPL9 protein (arrow).)

Western Blot (WB) (MRPL9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MRPL9 antibody detected the MRPL9 protein (arrow).)

Immunohistochemistry (IHC)

(MRPL9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPL9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (MRPL9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPL9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MRPL9 antibody
Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 39S subunit protein. A pseudogene
corresponding to this gene is found at 8q21.11. [provided by
RefSeq].
References
Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010) :
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
Suzuki, T., et al. J. Biol. Chem. 276(24):21724-21736(2001)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30243
NCBI Official Full Name
39S ribosomal protein L9, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L9
NCBI Official Symbol
MRPL9
NCBI Official Synonym Symbols
L9mt
NCBI Protein Information
39S ribosomal protein L9, mitochondrial
UniProt Protein Name
39S ribosomal protein L9, mitochondrial
Protein Family
UniProt Gene Name
MRPL9
UniProt Synonym Gene Names
L9mt; MRP-L9
UniProt Entry Name
RM09_HUMAN

NCBI Description

This is a nuclear gene encoding a protein component of the 39S subunit of the mitochondrial ribosome. Alternative splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Jul 2014]

Uniprot Description

MRPL9: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 8q21.11. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis

Research Articles on MRPL9

Similar Products

Product Notes

The MRPL9 mrpl9 (Catalog #AAA9214697) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 66-95. The MRPL9 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MRPL9 mrpl9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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