Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using p400 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit p400 Polyclonal Antibody | anti-EP400 antibody
p400 Antibody
Gene Names
EP400; P400; CAGH32; TNRC12
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
p400; Polyclonal Antibody; p400 Antibody; CAG repeat protein 32; CAGH 32; CAGH32; DKFZP434I225; Domino homolog; E1A binding protein p400; E1A-binding protein p400; EP 400; EP400; EP400_HUMAN; FLJ42018; FLJ45115; hDomino; KIAA1498; KIAA1818; p400 kDa SWI2/SNF2 related protein; p400 kDa SWI2/SNF2-related protein; P400 SWI2/SNF2 related protein; TNRC 12; TNRC12; Trinucleotide repeat containing 12; Trinucleotide repeat containing gene 12 protein; Trinucleotide repeat-containing gene 12 protein; anti-EP400 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
p400 Antibody detects endogenous levels of total p400.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
3123
Applicable Applications for anti-EP400 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human p400, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using p400 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using p400 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-EP400 antibody
Function: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
Subcellular Location: Nucleus.
Tissue Specificity: Ubiquitously expressed.
Subunit Structure: Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. May also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 interacts with TRRAP, RUVBL1 and RUVBL2. Component of a SWR1-like complex. Interacts with ZNF42. Interacts with PHF5A. Interacts with human cytomegalovirus UL27. Interacts with human adenovirus 5 E1A protein; this interaction stabilizes MYC (PubMed:18413597).
Similarity: Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Subcellular Location: Nucleus.
Tissue Specificity: Ubiquitously expressed.
Subunit Structure: Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. May also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 interacts with TRRAP, RUVBL1 and RUVBL2. Component of a SWR1-like complex. Interacts with ZNF42. Interacts with PHF5A. Interacts with human cytomegalovirus UL27. Interacts with human adenovirus 5 E1A protein; this interaction stabilizes MYC (PubMed:18413597).
Similarity: Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)343kD.
Predicted Molecular Weight: (Calculated)343kDa.
Predicted Molecular Weight: (Calculated)343kDa.
NCBI Official Full Name
E1A-binding protein p400
NCBI Official Synonym Full Names
E1A binding protein p400
NCBI Official Symbol
EP400
NCBI Official Synonym Symbols
P400; CAGH32; TNRC12
NCBI Protein Information
E1A-binding protein p400
UniProt Protein Name
E1A-binding protein p400
Protein Family
UniProt Gene Name
EP400
UniProt Synonym Gene Names
CAGH32; KIAA1498; KIAA1818; TNRC12; hDomino
UniProt Entry Name
EP400_HUMAN
Research Articles on EP400
Similar Products
Product Notes
The EP400 ep400 (Catalog #AAA9612249) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p400 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's p400 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the EP400 ep400 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p400, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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