Rabbit P2X7 Polyclonal Antibody | anti-P2X7 antibody

P2X7 Antibody

Cat. Num. AAA9613334

• Gene Names: P2RX7; P2X7
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (90%), Bovine (89%), Horse (100%), Sheep (89%), Dog (90%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: P2X7; Polyclonal Antibody; P2X7 Antibody; ATP receptor; P2rx7; P2RX7_HUMAN; P2X purinoceptor 7; P2Z receptor; Purinergic receptor; purinergic receptor P2X; ligand gated ion channel; 7; anti-P2X7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (90%), Bovine (89%), Horse (100%), Sheep (89%), Dog (90%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: P2X7 Antibody detects endogenous levels of total P2X7.; Tissue Specificity: Widely expressed with highest levels in brain and immune tissues.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-P2X7 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human P2X7, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
• Subunit Structure: Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB. Interacts (via C-terminus) with EMP2.
• Similarity: Belongs to the P2X receptor family.
• Subcellular Location: Cell membrane>Multi-pass membrane protein.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of P2X7 in lysates of Jurkat  , using P2X7 Antibody(AF4626).)

Related Product Information for anti-P2X7 antibody

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 300068987
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.3
• NCBI GenBank Nucleotide #: NM_002562.5
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: 68,585 Da
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• UniProt Protein Name: P2X purinoceptor 7
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7
• UniProt Entry Name: P2RX7_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Uniprot Description

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.

Protein type: Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; membrane; cell soma; integral to plasma membrane; cytoplasm; plasma membrane; terminal button; integral to nuclear inner membrane; intercellular junction; neuromuscular junction; lipid raft; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; lipopolysaccharide binding; magnesium ion binding; ATP binding; purinergic nucleotide receptor activity; ATP-gated cation channel activity; receptor binding

Biological Process: activation of MAPK activity; positive regulation of T cell mediated cytotoxicity; cytolysis; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; cell surface receptor linked signal transduction; multicellular organismal protein catabolic process; ceramide biosynthetic process; T cell homeostasis; positive regulation of cytolysis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; positive regulation of cytoskeleton organization and biogenesis; regulation of sodium ion transport; protein oligomerization; phagolysosome formation; phospholipid translocation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to mechanical stimulus; response to zinc ion; cell volume homeostasis; response to calcium ion; phospholipid transfer to membrane; cell morphogenesis; generation of action potential; regulation of killing of cells of another organism; T cell proliferation; plasma membrane organization and biogenesis; positive regulation of mitochondrial depolarization; pore complex biogenesis; inflammatory response; negative regulation of bone resorption; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; synaptic vesicle exocytosis; skeletal morphogenesis; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; innate immune response; protein processing; gene expression; membrane budding

Disease: Leukemia, Chronic Lymphocytic