Goat anti-Human P2RX7 Polyclonal Antibody | anti-P2RX7 antibody

P2RX7, NT (P2X Purinoceptor 7, P2X7, ATP Receptor, P2Z Receptor, Purinergic Receptor)

Cat. Num. AAA644383

• Gene Names: P2RX7; P2X7
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: P2RX7; Polyclonal Antibody; NT (P2X Purinoceptor 7; P2X7; ATP Receptor; P2Z Receptor; Purinergic Receptor); Anti -P2RX7; anti-P2RX7 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human P2RX7. Species sequence homology: canine, rat and mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-P2RX7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; ELISA: 1:16,000; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide YETNKVTRIQSMNY-C from the N-terminus of human P2RX7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(P2RX7 antibody staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-P2RX7 antibody

P2X7 is a member of a family of P2X receptors that are fast, ligand-gated cation channels that differ in the length of their intracellular C-termini. The long C-terminus of P2X7 allows time-dependent dilation of the channel, in response to ATP, to form a cytolytic pore allowing entry of large molecules. This receptor has been implicated in the lysis of antigen presenting cells by cytotoxic T lymphocytes, mitogenic stimulation of human T lymphocytes and the formation of multinucleated giant cells.

Product Categories/Family for anti-P2RX7 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 300068987
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.3
• NCBI GenBank Nucleotide #: NM_002562.5
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: 68,585 Da
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• UniProt Protein Name: P2X purinoceptor 7
• Protein Family: P2X purinoceptor
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7
• UniProt Entry Name: P2RX7_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Uniprot Description

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.

Protein type: Membrane protein, multi-pass; Receptor, misc.; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; membrane; cell soma; integral to plasma membrane; cytoplasm; plasma membrane; terminal button; integral to nuclear inner membrane; intercellular junction; neuromuscular junction; external side of plasma membrane; lipid raft

Molecular Function: protein binding; copper ion binding; protein homodimerization activity; zinc ion binding; lipopolysaccharide binding; magnesium ion binding; ATP binding; purinergic nucleotide receptor activity; receptor binding; ATP-gated cation channel activity

Biological Process: activation of MAPK activity; positive regulation of T cell mediated cytotoxicity; cytolysis; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; cell surface receptor linked signal transduction; multicellular organismal protein catabolic process; ceramide biosynthetic process; positive regulation of cytolysis; T cell homeostasis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; positive regulation of cytoskeleton organization and biogenesis; regulation of sodium ion transport; protein oligomerization; phagolysosome formation; phospholipid translocation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to mechanical stimulus; response to zinc ion; cell volume homeostasis; response to calcium ion; phospholipid transfer to membrane; cell morphogenesis; generation of action potential; regulation of killing of cells of another organism; T cell proliferation; plasma membrane organization and biogenesis; positive regulation of mitochondrial depolarization; pore complex biogenesis; negative regulation of bone resorption; inflammatory response; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; synaptic vesicle exocytosis; homeostasis of number of cells within a tissue; skeletal morphogenesis; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; innate immune response; protein processing; gene expression; membrane budding

Disease: Leukemia, Chronic Lymphocytic