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Western Blot (WB) (p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)

Rabbit anti-Human p21WAF1 Polyclonal Antibody | anti-LCMT2 antibody

p21WAF1 Antibody (Center H450)

Gene Names
LCMT2; PPM2; TYW4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
p21WAF1; Polyclonal Antibody; p21WAF1 Antibody (Center H450); tRNA wybutosine-synthesizing protein 4; tRNA yW-synthesizing protein 4; Leucine carboxyl methyltransferase 2; tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase; LCMT2; KIAA0547; TYW4; anti-LCMT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human p21WAF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.39 (varies by lot)
Sequence Positions
435-463
Sequence Length
686
Applicable Applications for anti-LCMT2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)

Western Blot (WB) (p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)
Related Product Information for anti-LCMT2 antibody
The protein encoded by this intronless gene belongs to the
highly variable methyltransferase superfamily. This gene is the
inferred homolog of the Saccharomyces cerevisiae
carboxymethyltransferase gene PPM2 that is essential for the
synthesis of the hypermodified guanosine Wybutosine (yW). [provided
by RefSeq].
Product Categories/Family for anti-LCMT2 antibody
References
Noma, A., et al. EMBO J. 25(10):2142-2154(2006)
De Baere, I., et al. Biochemistry 38(50):16539-16547(1999)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75602
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 4
NCBI Official Synonym Full Names
leucine carboxyl methyltransferase 2
NCBI Official Symbol
LCMT2
NCBI Official Synonym Symbols
PPM2; TYW4
NCBI Protein Information
tRNA wybutosine-synthesizing protein 4
UniProt Protein Name
tRNA wybutosine-synthesizing protein 4
UniProt Gene Name
LCMT2
UniProt Synonym Gene Names
KIAA0547; TYW4; tRNA yW-synthesizing protein 4
UniProt Entry Name
TYW4_HUMAN

NCBI Description

The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008]

Uniprot Description

LCMT2: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family.

Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 2.3.1.231; Methyltransferase; Lipid Metabolism - androgen and estrogen; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; EC 2.1.1.290

Chromosomal Location of Human Ortholog: 15q15.3

Molecular Function: methyltransferase activity; protein binding

Biological Process: methylation; tRNA processing

Research Articles on LCMT2

Similar Products

Product Notes

The LCMT2 lcmt2 (Catalog #AAA9206507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 435-463. The p21WAF1 Antibody (Center H450) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p21WAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the LCMT2 lcmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21WAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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