Western Blot (WB)
(p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)
Rabbit anti-Human p21WAF1 Polyclonal Antibody | anti-p21WAF1 antibody
p21WAF1, ID (H450) (LCMT2, KIAA0547, TYW4, Leucine carboxyl methyltransferase 2, p21WAF1/CIP1 promoter-interacting protein, tRNA wybutosine-synthesizing protein 4 homolog) (AP)
Gene Names
LCMT2; PPM2; TYW4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
p21WAF1; Polyclonal Antibody; ID (H450) (LCMT2; KIAA0547; TYW4; Leucine carboxyl methyltransferase 2; p21WAF1/CIP1 promoter-interacting protein; tRNA wybutosine-synthesizing protein 4 homolog) (AP); LCMT2; tRNA wybutosine-synthesizing protein 4 homolog; anti-p21WAF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-p21WAF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the Central region of human p21WAF1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)
Western Blot (WB)
(p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)
Related Product Information for anti-p21WAF1 antibody
The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq].
Product Categories/Family for anti-p21WAF1 antibody
References
Noma, A., et al. EMBO J. 25(10):2142-2154(2006). De Baere, I., et al. Biochemistry 38(50):16539-16547(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,602 Da
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 4
NCBI Official Synonym Full Names
leucine carboxyl methyltransferase 2
NCBI Official Symbol
LCMT2
NCBI Official Synonym Symbols
PPM2; TYW4
NCBI Protein Information
tRNA wybutosine-synthesizing protein 4; tRNA yW-synthesizing protein 4; tRNA-yW synthesizing protein 4; tRNA-Wybutosine-synthesizing protein 4; p21WAF1/CIP1 promoter-interacting protein; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransfera
UniProt Protein Name
tRNA wybutosine-synthesizing protein 4
UniProt Gene Name
LCMT2
UniProt Synonym Gene Names
KIAA0547; TYW4; tRNA yW-synthesizing protein 4
UniProt Entry Name
TYW4_HUMAN
Uniprot Description
LCMT2: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family.
Protein type: Lipid Metabolism - androgen and estrogen; Amino Acid Metabolism - histidine; Methyltransferase; Amino Acid Metabolism - tyrosine; EC 2.3.1.231; EC 2.1.1.290; Other Amino Acids Metabolism - selenoamino acid
Chromosomal Location of Human Ortholog: 15q15.3
Molecular Function: methyltransferase activity; protein binding
Biological Process: methylation; tRNA processing
Similar Products
Product Notes
The p21WAF1 lcmt2 (Catalog #AAA6330120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p21WAF1, ID (H450) (LCMT2, KIAA0547, TYW4, Leucine carboxyl methyltransferase 2, p21WAF1/CIP1 promoter-interacting protein, tRNA wybutosine-synthesizing protein 4 homolog) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p21WAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the p21WAF1 lcmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21WAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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