Rabbit anti-Human p21WAF1 Polyclonal Antibody | anti-Kcnh8 antibody

p21WAF1, ID (H450) (LCMT2, KIAA0547, TYW4, Leucine carboxyl methyltransferase 2, p21WAF1/CIP1 promoter-interacting protein, tRNA wybutosine-synthesizing protein 4 homolog)

Cat. Num. AAA6000359

• Gene Names: Kcnh8; ELK3; Elk1; Ets-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: p21WAF1; Polyclonal Antibody; ID (H450) (LCMT2; KIAA0547; TYW4; Leucine carboxyl methyltransferase 2; p21WAF1/CIP1 promoter-interacting protein; tRNA wybutosine-synthesizing protein 4 homolog); Anti -p21WAF1; anti-Kcnh8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Kcnh8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the Central region of human p21WAF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(p21WAF1 Antibody (Center H450) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).)

Related Product Information for anti-Kcnh8 antibody

The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq].

Product Categories/Family for anti-Kcnh8 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 21489989
• NCBI GeneID: 246325
• NCBI Accession #: NP_659563.1
• NCBI GenBank Nucleotide #: NM_145095.1
• UniProt Accession #: Q9QWS8; O88877
• Molecular Weight: 123,231 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily H member 8
• NCBI Official Synonym Full Names: potassium voltage-gated channel, subfamily H (eag-related), member 8
• NCBI Official Symbol: Kcnh8
• NCBI Official Synonym Symbols: ELK3; Elk1; Ets-1
• NCBI Protein Information: potassium voltage-gated channel subfamily H member 8; ELK channel 3; ether-a-go-go-like potassium channel 1; ether-a-go-go-like potassium channel 3; voltage-gated potassium channel subunit Kv12.1; potassium voltage-gated channel, subfamily H, member 8
• UniProt Protein Name: Potassium voltage-gated channel subfamily H member 8
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcnh8
• UniProt Synonym Gene Names: Elk1; Elk3; ELK channel 3
• UniProt Entry Name: KCNH8_RAT

Uniprot Description

Function: Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary. Ref.3 Ref.4

Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities: Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.1/KCNH8 sub-subfamily. [View classification]Contains 1 cyclic nucleotide-binding domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 1 PAS (PER-ARNT-SIM) domain.