Rat P21 Protein (P21) ELISA Kit | CDKN1A elisa kit

Rat P21 Protein, P21 ELISA Kit

Cat. Num. AAA2602846

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Rat
• Synonyms: P21 Protein (P21); Rat P21 Protein; P21 ELISA Kit; CDKN1A elisa kit

• Reactivity: Rat

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDKN1A elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat P21 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 453135
• NCBI GeneID: 1026
• UniProt Accession #: P38936; Q14010; Q6FI05; Q9BUT4
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: p21
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1; DNA synthesis inhibitor; CDK-interacting protein 1; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Inhibitor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; cyclin binding; cyclin-dependent protein kinase activating kinase activity; metal ion binding; ubiquitin protein ligase binding; protein complex binding

Biological Process: nerve growth factor receptor signaling pathway; response to arsenic; response to toxin; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; cell cycle arrest; G2/M transition of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to corticosterone stimulus; response to X-ray; response to drug; epidermal growth factor receptor signaling pathway; organ regeneration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; regulation of protein import into nucleus, translocation; innate immune response; negative regulation of phosphorylation; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle