Mouse anti-Human CD25 Monoclonal Antibody | anti-CD25 antibody

anti-human CD25-Biotin

Cat. Num. AAA666622

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: CD25; Monoclonal Antibody; anti-human CD25-Biotin; Monoclonal anti-human CD25(IL2-R alpha; TAC)/Biotin; anti-CD25 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7G7B6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-CD25 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Performance: Cultured human CEM cells were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD25/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptaviden/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.88 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD25 antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Stimulated PBMC
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD25 antibody

Information: Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4). Antibody 7G7B6 recognizes epitope B of the IL-2 alpha receptor of about 55 kd (1,6). Antibody 7G7B6 does not block IL-2 binding to its receptor (1).

References

(1) L.A. Rubin, et al, (1985) Hybidoma 4: 91-102. (2) B. Boutin, et al, (1989) Neuropediatrics 20: 202-206. (3) T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911. (4) T.A. Waldmann, et al, (1995) Blood 86: 4063-4075. (5) U.S. Patent No. 4,707,443. (6)Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 403-406.

NCBI and Uniprot Product Information

• NCBI GI #: 55904
• UniProt Accession #: P25094
• Molecular Weight: 17,946 Da
• NCBI Official Full Name: CD25
• UniProt Protein Name: Peripheral myelin protein 22
• UniProt Gene Name: Pmp22
• UniProt Synonym Gene Names: Cd25; Pmp-22; PMP-22; SAG
• UniProt Entry Name: PMP22_RAT

Uniprot Description

Might be involved in growth regulation, and in myelinization in the peripheral nervous system.