Western Blot (WB)
(Western blot analysis of p14 ARF expression in Saos2 (A); Molt (B); K562 (C); Jurkat (D); HL60 (E); MCF7 (F); HeLa (G) whole cell lysates.)
Rabbit p14 ARF Polyclonal Antibody | anti-p14ARF antibody
p14 ARF Antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen Affinity Chromatography
Synonyms
p14 ARF; Polyclonal Antibody; p14 ARF Antibody; Purified Polyclonal p14 ARF Antibody; Polyclonal p14 ARF antibody; Anti-p14 ARF antibody; CDKN2 antibody; MLM antibody; Cyclin-dependent kinase inhibitor 2A isoform 4 antibody; p14ARF antibody; p19ARF antibody; anti-p14ARF antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of p14 ARF protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
131
Applicable Applications for anti-p14ARF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
Immunogen
p14 ARF antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p14 ARF as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of p14 ARF expression in Saos2 (A); Molt (B); K562 (C); Jurkat (D); HL60 (E); MCF7 (F); HeLa (G) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p14 ARF expression in Saos2 (A); Molt (B); K562 (C); Jurkat (D); HL60 (E); MCF7 (F); HeLa (G) whole cell lysates.)
Related Product Information for anti-p14ARF antibody
p14 ARF is induced in response to elevated mitogenic stimulation, such as aberrant growth signaling from MYC and Ras (protein). It accumulates mainly in the nucleolus where it forms stable complexes with NPM or Mdm2. These interactions allow p14ARF to act as a tumor suppressor by inhibiting ribosome biogenesis or initiating p53-dependent cell cycle arrest and apoptosis, respectively.
Product Categories/Family for anti-p14ARF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
p14ARF protein
Similar Products
Product Notes
The p14ARF (Catalog #AAA5314150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p14 ARF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IHC: 1:100-1:200 IF: 1:100-1:500 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the p14ARF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p14 ARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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