Mouse anti-Human Phosphodiesterase 5A Monoclonal Antibody | anti-PDE5A antibody

Phosphodiesterase 5A (PDE5A, cGMP-Specific 3',5'-Cyclic Phosphodiesterase, cGMP-Binding cGMP-Specific Phosphodiesterase, CGB-PDE, PDE5) (MaxLight 405)

Cat. Num. AAA6191787

• Gene Names: PDE5A; CN5A; PDE5; CGB-PDE
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Phosphodiesterase 5A; Monoclonal Antibody; Phosphodiesterase 5A (PDE5A; cGMP-Specific 3'; 5'-Cyclic Phosphodiesterase; cGMP-Binding cGMP-Specific Phosphodiesterase; CGB-PDE; PDE5) (MaxLight 405); anti-PDE5A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 9H5
• Specificity: Recognizes human Phosphodiesterase 5A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PDE5A antibody: FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa29-138 from human Phosphodiesterase 5A with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP*
• Conjugate: MaxLight405
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against immunogen (38.21kD).)

Testing Data

(Detection limit for is 1ng/ml as a capture antibody.)

Related Product Information for anti-PDE5A antibody

Phosphodiesterase 5A, a cGMP-specific phosphodiesterase, has been implicated in primary pulmonary hypertension, cardiac failure and erectile dysfunction. PDE5A also may function in the regulation of coronary blood flow. PDE5A is the predominant enzyme responsible for cGMP hydrolysis in trabecular smooth muscle. PDE5A activitity is inhibited by sildenafil (Viagra) and zaprinast. At least three alternatively spliced transcripts have been reported.

Product Categories/Family for anti-PDE5A antibody

Antibodies; Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 61744435
• NCBI GeneID: 8654
• NCBI Accession #: NP_001074
• NCBI GenBank Nucleotide #: NM_001083
• UniProt Accession #: O76074; O75026; O75887; Q86UI0; Q86V66; Q9Y6Z6; A0AV69; A8K2C4
• Molecular Weight: 94,805 Da
• NCBI Official Full Name: cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 5A
• NCBI Official Symbol: PDE5A
• NCBI Official Synonym Symbols: CN5A; PDE5; CGB-PDE
• NCBI Protein Information: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Protein Name: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE5A
• UniProt Synonym Gene Names: PDE5; CGB-PDE

NCBI Description

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding

Biological Process: cGMP catabolic process