Western Blot (WB)
(The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.)
Rabbit anti-Human P100 Polyclonal Antibody | anti-TPX2 antibody
P100 Antibody
Gene Names
TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
P100; Polyclonal Antibody; P100 Antibody; Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519; anti-TPX2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This P100 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-382 amino acids from human P100.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
350-382
Sequence Length
783
Applicable Applications for anti-TPX2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.)
Western Blot (WB)
(The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-TPX2 antibody
The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.
Product Categories/Family for anti-TPX2 antibody
References
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Heidebrecht, H.J., et al., Mol. Cancer Res. 1(4):271-279 (2003).
Garrett, S., et al., Curr. Biol. 12(23):2055-2059 (2002).
Gruss, O.J., et al., Nat. Cell Biol. 4(11):871-879 (2002).
Kufer, T.A., et al., J. Cell Biol. 158(4):617-623 (2002).
Heidebrecht, H.J., et al., Mol. Cancer Res. 1(4):271-279 (2003).
Garrett, S., et al., Curr. Biol. 12(23):2055-2059 (2002).
Gruss, O.J., et al., Nat. Cell Biol. 4(11):871-879 (2002).
Kufer, T.A., et al., J. Cell Biol. 158(4):617-623 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85653
NCBI Official Full Name
targeting protein for Xklp2
NCBI Official Synonym Full Names
TPX2, microtubule-associated
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
NCBI Protein Information
targeting protein for Xklp2
UniProt Protein Name
Targeting protein for Xklp2
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
Uniprot Description
TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.
Protein type: Cell cycle regulation; Microtubule-binding
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: microtubule cytoskeleton; axon hillock; nucleoplasm; spindle pole; microtubule; cytoplasm; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: mitosis; cell proliferation; cell division; apoptosis; activation of protein kinase activity
Research Articles on TPX2
Similar Products
Product Notes
The TPX2 tpx2 (Catalog #AAA9211097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 350-382. The P100 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P100 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P100, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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