Western Blot (WB)
(Western analysis of extracts from Jurkat cells using P100 antibody.)
Mouse anti-Human, mouse P100 Monoclonal Antibody | anti-TPX2 antibody
P100 Antibody
Gene Names
TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
P100; Monoclonal Antibody; P100 Antibody; Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519; anti-TPX2 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
19AT738.292
Specificity
This monoclonal antibody is generated from mice immunized with four KLH conjugated synthetic peptides selected from human P100.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Length
747
Applicable Applications for anti-TPX2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:100~500
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western analysis of extracts from Jurkat cells using P100 antibody.)
Western Blot (WB)
(Western analysis of extracts from Jurkat cells using P100 antibody.)
Product Categories/Family for anti-TPX2 antibody
References
Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.
A cancer-associated aurora A mutant is mislocalized and misregulated due to loss of interaction with TPX2. Bibby RA, et al. J Biol Chem, 2009 Nov 27. PMID 19801554.
Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.
Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas. Shigeishi H, et al. Int J Oncol, 2009 Jun. PMID 19424574.
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase. Moss DK, et al. J Cell Sci, 2009 Mar 1. PMID 19208764.
A cancer-associated aurora A mutant is mislocalized and misregulated due to loss of interaction with TPX2. Bibby RA, et al. J Biol Chem, 2009 Nov 27. PMID 19801554.
Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.
Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas. Shigeishi H, et al. Int J Oncol, 2009 Jun. PMID 19424574.
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase. Moss DK, et al. J Cell Sci, 2009 Mar 1. PMID 19208764.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85653
NCBI Official Full Name
targeting protein for Xklp2
NCBI Official Synonym Full Names
TPX2, microtubule-associated
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
NCBI Protein Information
targeting protein for Xklp2
UniProt Protein Name
Targeting protein for Xklp2
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
Uniprot Description
TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.
Protein type: Microtubule-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; axon hillock; microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: cell proliferation; mitosis; cell division; apoptosis; activation of protein kinase activity
Research Articles on TPX2
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Product Notes
The TPX2 tpx2 (Catalog #AAA9200624) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The P100 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's P100 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:100~500. Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P100, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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