Rabbit anti-Rhesus P-Selectin Polyclonal Antibody | anti-SELP antibody

Anti-P-Selectin Antibody, Rabbit Polyclonal

Cat. Num. AAA8113229

• Reactivity: Rhesus
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: P-Selectin; Polyclonal Antibody; Anti-P-Selectin Antibody; Rabbit Polyclonal; SELP/P Selectin Antibody; Rabbit PAb; Antigen Affinity Purified; selectin P (granule membrane protein 140kDa; antigen CD62); anti-SELP antibody

• Host: Rabbit
• Reactivity: Rhesus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rhesus SELP/P Selectin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SELP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Rhesus SELP/P Selectin protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rhesus SELP/P Selectin. SELP/P Selectin specific IgG was purified by Rhesus SELP/P Selectin affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SELP rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:91 kDaObserved band size:91 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SELP antibody

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

References

Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.

NCBI and Uniprot Product Information

• Protein Family: P-selectin