Rabbit anti-Human Ovalbumin Polyclonal Antibody | anti-OVAL antibody

Rabbit anti Ovalbumin Polyclonal Antibody

Cat. Num. AAA460398

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Ovalbumin; Polyclonal Antibody; Rabbit anti Ovalbumin Polyclonal Antibody; SERPINB14; anti-OVAL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~45 kDa of chicken egg white Ovomucoid protein
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 386

• Applicable Applications for anti-OVAL antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the 181-200aa of chicken egg white Ovalbumin protein.
• Positive Control: Ovalbumin
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

Related Product Information for anti-OVAL antibody

Ovalbumin is a major protein isolated from chicken egg white. It is a 386 aa secreted protein. Allergy to chicken egg or proteins is one of the most frequent causes of food hypersensitivity in infants and young children. Both IgG and IgA class antibodies may be detected. Ovalbumin intolerance has been implicated in a number of conditions affecting children. In particular, children with cystic fibrosis show elevated anti-Ovalbumin antibodies. Ovalbumin antibodies have also been noted in some forms of kidney disease. A relationship between food allergy and infantile autism has also been observed. Children with insulin-dependent diabetes mellitus show an enhanced immune response to both beta- actoglobulin and ovalbumin, a phenomenon that may be related to the development of the disease. Conditions related to ovalbumin intolerance usually resolve once egg and egg based foods have been withdrawn from the patient's diet. Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk. The major proteins of chicken eggs are: Ovalbumin (45 kda, 54%), Conalbumin (13%, 80 kda), Ovomucoid (11%, 28 kda), Lysozyme (3.5%, 14 kda), and other proteins.

References

Henderson, J.Y., Moir, A.J., Fothergill, L.A. and Fothergill, J.E. Sequences of sixteen phosphoserine peptides from ovalbumins of eight species. Eur. J. Biochem. 114 (2), 439-450 (1981).

NCBI and Uniprot Product Information

• NCBI GI #: 906366229
• NCBI GeneID: 101801591
• NCBI Accession #: NP_001298098.1
• NCBI GenBank Nucleotide #: NM_001311169.1
• Molecular Weight: ~45 kDa
• NCBI Official Full Name: ovalbumin
• NCBI Official Symbol: OVAL
• NCBI Protein Information: ovalbumin
• Protein Family: Ovalbumin