Rabbit anti-Human OSTF1 Polyclonal Antibody | anti-OSTF1 antibody

OSTF1, NT (OSTF1, Osteoclast-stimulating factor 1) (FITC)

Cat. Num. AAA6329782

• Gene Names: OSTF1; OSF; SH3P2; bA235O14.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OSTF1; Polyclonal Antibody; NT (OSTF1; Osteoclast-stimulating factor 1) (FITC); Osteoclast-stimulating factor 1; anti-OSTF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OSTF1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-OSTF1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-37 in the N-terminal region of human OSTF1, UniProt Accession #NP_036515.4; Q92885.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in U251 cell line lysates (35ug/lane) usingMBS6007043. This demonstrates MBS6007043 detected the OSTF1 protein (arrow).)

Related Product Information for anti-OSTF1 antibody

Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).

Product Categories/Family for anti-OSTF1 antibody

Antibodies; Proteins

References

Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010): Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Tong, S., et al. Proteins 75(1):245-251(2009). Voss, M., et al. BMC Immunol. 10, 53 (2009): Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 166235148
• NCBI GeneID: 26578
• NCBI Accession #: NP_036515.4
• NCBI GenBank Nucleotide #: NM_012383.4
• UniProt Accession #: Q92882; Q5W126; Q96IJ4
• Molecular Weight: 23,787 Da
• NCBI Official Full Name: osteoclast-stimulating factor 1
• NCBI Official Synonym Full Names: osteoclast stimulating factor 1
• NCBI Official Symbol: OSTF1
• NCBI Official Synonym Symbols: OSF; SH3P2; bA235O14.1
• NCBI Protein Information: osteoclast-stimulating factor 1
• UniProt Protein Name: Osteoclast-stimulating factor 1
• Protein Family: Osteoclast-stimulating factor
• UniProt Gene Name: OSTF1
• UniProt Entry Name: OSTF1_HUMAN

NCBI Description

Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]

Uniprot Description

OSTF1: Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q13-q21.2

Cellular Component: cytoplasm; intracellular

Molecular Function: protein binding; SH3 domain binding

Biological Process: ossification; signal transduction