Rabbit anti-Human, Mouse PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5 (Protein arginine N Methyltransferase 5, Shk1 Kinase-binding Protein 1 Homolog, Jak-binding Protein 1, JBP1) (MaxLight 405)

Cat. Num. AAA6494440

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRMT5; Polyclonal Antibody; PRMT5 (Protein arginine N Methyltransferase 5; Shk1 Kinase-binding Protein 1 Homolog; Jak-binding Protein 1; JBP1) (MaxLight 405); anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT5, Mr ~70kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PRMT5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa60-79, KNRPGPQTRSDLLLSGRDWN, of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis: Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS614939 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT5 (70kD).)

Related Product Information for anti-PRMT5 antibody

Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein. PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.

Product Categories/Family for anti-PRMT5 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Branscombe, T.L., et al., J. Biol. Chem. 276: 32971-32976, 2001. 2. Rho, J., et al., J. Biol. Chem. 276: 11393-11401, 2001. 3. Boisvert, F.M., et al., J. Cell Biol. 159: 957-969, 2002. 4. Pollack, B.P., et al., J. Biol. Chem. 274: 31531-31542, 1999. 5. Friesen, W.J., et al., Mol. Cell. Biol. 21: 8289-3300, 2001. 6. Meister, G., et al., Curr. Biol. 11: 1990-1994, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 32171585
• NCBI GeneID: 10419
• NCBI Accession #: O14744.4
• NCBI GenBank Nucleotide #: NM_006109
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 637
• NCBI Official Full Name: Protein arginine N-methyltransferase 5
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation