Goat ORP5 Polyclonal Antibody | anti-OSBPL5 antibody

Goat anti-ORP5 (OBPH1) Antibody

Cat. Num. AAA421287

• Gene Names: OSBPL5; ORP5; OBPH1
• Reactivity: Tested: Human;; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ORP5; Polyclonal Antibody; Goat anti-ORP5 (OBPH1) Antibody; OSBPL5; OBPH1; FLJ31948; KIAA1534; DKFZp586H1719; oxysterol binding protein-like 5; OSBP-related protein 5; oxysterol-binding protein homologue 1; oxysterol-binding protein-related protein 5; FLJ42929; oxysterol-binding protein-like protein 5; OSBPL5 antibody; ORP5 antibody; OBPH1 antibody; FLJ31948 antibody; KIAA1534 antibody; DKFZp586H1719 antibody; oxysterol binding protein-like 5 antibody; OSBP-related protein 5 antibody; oxysterol-binding protein homologue 1 antibody; oxysterol-binding protein-related protein 5 antibody; FLJ42929 antibody; oxysterol-binding protein-like protein 5 antibody; ORP5 (OBPH1); anti-OSBPL5 antibody

• Host: Goat
• Reactivity: Tested: Human;; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KEEAFLRRRFSLC
• Sequence Length: 879

• Applicable Applications for anti-OSBPL5 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 110kDa band observed in human brain (cerebellum) and spleen lysates (calculated MW of 98.4kDa according to NP_065947.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.; IHC: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 ug/ml.
• Immunogen: Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1; NP_663613.1; NP_001137535.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(MBS421287 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry (IHC)

(MBS421287 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 14149740
• NCBI GeneID: 114879
• NCBI Accession #: NP_065947.1; NP_663613.1; NP_001137535.1
• NCBI GenBank Nucleotide #: NM_020896.3
• NCBI Official Full Name: oxysterol-binding protein-related protein 5 isoform a
• NCBI Official Synonym Full Names: oxysterol binding protein like 5
• NCBI Official Symbol: OSBPL5
• NCBI Official Synonym Symbols: ORP5; OBPH1
• NCBI Protein Information: oxysterol-binding protein-related protein 5
• UniProt Protein Name: Oxysterol-binding protein-related protein 5
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL5
• UniProt Synonym Gene Names: ORP-5; OSBP-related protein 5
• UniProt Entry Name: OSBL5_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that play a key role in the maintenance of cholesterol balance in the body. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. This gene has been shown to be imprinted, with preferential expression from the maternal allele only in placenta. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

Uniprot Description

OSBPL5: May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytosol; endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: cholesterol binding; oxysterol binding; phosphatidylserine binding; phospholipid transporter activity

Biological Process: cholesterol metabolic process; cholesterol transport; Golgi to plasma membrane transport; phospholipid transport