Western Blot (WB)
((0.6ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TIP30/CC3 Polyclonal Antibody | anti-HTATIP2 antibody
Goat anti-TIP30/CC3 Antibody
Gene Names
HTATIP2; CC3; TIP30; SDR44U1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TIP30/CC3; Polyclonal Antibody; Goat anti-TIP30/CC3 Antibody; HTATIP2; CC3; TIP30; HIV-1 Tat interactive protein 2; 30kDa; Tat-interacting protein (30kD); 30 kD; 30 kDa; FLJ26963; SDR44U1; short chain dehydrogenase/reductase family 44U; member 1; CC3 antibody; TIP30 antibody; HTATIP2 antibody; 30kDa antibody; Tat-interacting protein (30kD) antibody; 30 kD antibody; 30 kDa antibody; FLJ26963 antibody; SDR44U1 antibody; member 1 antibody; TIP30 / CC3; anti-HTATIP2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
AETEALSKLREDFR
Sequence Length
276
Applicable Applications for anti-HTATIP2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 28kDa band observed in lysates of cell line HeLa (calculated MW of 27. 0kDa according to NP_006401.3). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 28kDa band observed in lysates of cell line HeLa (calculated MW of 27. 0kDa according to NP_006401.3). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence AETEALSKLREDFR-C, from the N Terminus of the protein sequence according to NP_001091990.1; NP_006401.3; NP_001091991.1; NP_001091992.1; NP_001091993.1.
Epitope
N Terminus
Note
This antibody is expected to recognise all three reported isoforms. Variants (NP_006401.3; NP_001091991.1; NP_001091992.1) encode the same isoform.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.6ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.6ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,131 Da
NCBI Official Full Name
oxidoreductase HTATIP2 isoform a
NCBI Official Synonym Full Names
HIV-1 Tat interactive protein 2
NCBI Official Symbol
HTATIP2
NCBI Official Synonym Symbols
CC3; TIP30; SDR44U1
NCBI Protein Information
oxidoreductase HTATIP2
UniProt Protein Name
Oxidoreductase HTATIP2
Protein Family
UniProt Gene Name
HTATIP2
UniProt Entry Name
HTAI2_HUMAN
Uniprot Description
HTATIP2: Oxidoreductase required for tumor suppression. NAPDH- bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Tumor suppressor; Oxidoreductase; EC 1.1.1.-; Nuclear envelope; Apoptosis
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: cytoplasm; membrane; nuclear envelope; nucleoplasm; nucleus
Molecular Function: oxidoreductase activity; protein binding; transcription coactivator activity
Biological Process: angiogenesis; apoptosis; cell differentiation; negative regulation of apoptosis; nuclear import; regulation of angiogenesis; regulation of transcription from RNA polymerase II promoter; viral reproduction
Research Articles on HTATIP2
Similar Products
Product Notes
The HTATIP2 htatip2 (Catalog #AAA420235) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TIP30/CC3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIP30/CC3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 28kDa band observed in lysates of cell line HeLa (calculated MW of 27. 0kDa according to NP_006401.3). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the HTATIP2 htatip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AETEALSKLR EDFR. It is sometimes possible for the material contained within the vial of "TIP30/CC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
