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Western Blot (WB) (Western Blot (WB) analysis of OGDH polyclonal antibody)

Rabbit OGDH Polyclonal Antibody | anti-OGDH antibody

OGDH Polyclonal Antibody

Gene Names
OGDH; E1k; KGD1; OGDC; AKGDH; OGDH2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
OGDH; Polyclonal Antibody; OGDH Polyclonal Antibody; 2-oxoglutarate dehydrogenase; mitochondrial; 2-oxoglutarate dehydrogenase complex component E1; OGDC-E1; Alpha-ketoglutarate dehydrogenase; anti-OGDH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
OGDH polyclonal antibody detects endogenous levels of OGDH protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1023
Applicable Applications for anti-OGDH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human OGDH.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of OGDH polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of OGDH polyclonal antibody)
Related Product Information for anti-OGDH antibody
The alpha-ketoglutarate dehydrogenase (alpha-KGD) complex is a multienzyme complex which localizes to the mitochondrial matrix and consists of three protein subunits: alpha-ketoglutarate dehydrogenase, also designated alpha-KGD, E1k or oxoglutarate dehydrogenase (OGDH); dihydrolipoyl succinyltransferase (E2k or DLST); and dihydrolipoyl dehydrogenase (E3). The alpha-KGD subunit of the alpha-KGD complex catalyzes the conversion of alpha-ketoglutarate to succinyl-CoA and CO2, an essential reaction of the tricarboxylic acid cycle. A definciency in alpha-KGD results in hypotonia, metabolic acidosis, hyperlactatemia immediately after birth, and neurologic deterioration resulting in death at about 30 months of age. Low molar ratios of ketone bodies in plasma of neonates with congenital lactic acidosis are proposed indicators of tricarboxylic acid cycle dysfunction.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 116kDa
NCBI Official Full Name
2-oxoglutarate dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
oxoglutarate dehydrogenase
NCBI Official Symbol
OGDH
NCBI Official Synonym Symbols
E1k; KGD1; OGDC; AKGDH; OGDH2
NCBI Protein Information
2-oxoglutarate dehydrogenase, mitochondrial
UniProt Protein Name
2-oxoglutarate dehydrogenase, mitochondrial
UniProt Gene Name
OGDH
UniProt Synonym Gene Names
OGDC-E1
UniProt Entry Name
ODO1_HUMAN

NCBI Description

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Uniprot Description

OGDH: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 1.2.4.2

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; cytosol; oxoglutarate dehydrogenase complex

Molecular Function: chaperone binding; metal ion binding; heat shock protein binding; thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity

Biological Process: generation of precursor metabolites and energy; glycolysis; striatum development; succinyl-CoA metabolic process; tricarboxylic acid cycle; hippocampus development; pyramidal neuron development; thalamus development; 2-oxoglutarate metabolic process; cellular metabolic process; cerebellar cortex development; tangential migration from the subventricular zone to the olfactory bulb; NADH metabolic process; lysine catabolic process

Disease: Alpha-ketoglutarate Dehydrogenase Deficiency

Research Articles on OGDH

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Product Notes

The OGDH ogdh (Catalog #AAA3011541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OGDH Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OGDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the OGDH ogdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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