Rabbit anti-Human PRMT3 Polyclonal Antibody | anti-PRMT3 antibody

PRMT3, CT (Protein Arginine N-Methyltransferase 3) (Azide free) (HRP)

Cat. Num. AAA6494397

• Gene Names: PRMT3; HRMT1L3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRMT3; Polyclonal Antibody; CT (Protein Arginine N-Methyltransferase 3) (Azide free) (HRP); anti-PRMT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRMT3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRMT3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PRMT3 antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 44771198
• NCBI GeneID: 10196
• NCBI Accession #: NP_005779.1
• NCBI GenBank Nucleotide #: NM_005788.3
• UniProt Accession #: O60678; B4DUC7
• Molecular Weight: 52,875 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 3 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 3
• NCBI Official Symbol: PRMT3
• NCBI Official Synonym Symbols: HRMT1L3
• NCBI Protein Information: protein arginine N-methyltransferase 3
• UniProt Protein Name: Protein arginine N-methyltransferase 3
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT3
• UniProt Synonym Gene Names: HRMT1L3
• UniProt Entry Name: ANM3_HUMAN

Uniprot Description

PRMT3: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Belongs to the protein arginine N-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; EC 2.1.1.-; Methyltransferase; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol

Molecular Function: histone-arginine N-methyltransferase activity; methyltransferase activity; protein binding; protein-arginine omega-N asymmetric methyltransferase activity

Biological Process: negative regulation of protein ubiquitination; regulation of transcription, DNA-dependent