Rabbit anti-Human OCT4 Polyclonal Antibody | anti-OCT4 antibody

OCT4, NT (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3) (MaxLight 750)

Cat. Num. AAA6327588

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: OCT4; Polyclonal Antibody; NT (POU5F1; OCT3; OTF3; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3) (MaxLight 750); POU5F1; Octamer-binding transcription factor 3; anti-OCT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OCT4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-OCT4 antibody: Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human OCT4, UniProt Accession #Q01860
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot to detect OCT4 (OCT3) in A375 cell lysate usingMBS6003335.)

Immunofluorescence (IF)

(Immunofluorescence analysis in HeLa cells using MBS6003335 (0.025mg/ml) was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Related Product Information for anti-OCT4 antibody

Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.

Product Categories/Family for anti-OCT4 antibody

Antibodies; Transcription Factors

References

1. Looijenga, L.H., et al., Cancer Res. 63(9):2244-2250 (2003). 2. Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). 3. Nichols, J., et al., Cell 95(3):379-391 (1998). 4. Crouau-Roy, B., et al., Genomics 21(1):241-243 (1994). 5. Takeda, J., et al., Nucleic Acids Res. 20(17):4613-4620 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 42560248
• NCBI GeneID: 5460
• NCBI Accession #: NP_002692
• NCBI GenBank Nucleotide #: NM_002701.5
• UniProt Accession #: Q01860; P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
• Molecular Weight: 30,085 Da
• NCBI Official Full Name: POU domain, class 5, transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; POU-type homeodomain-containing DNA-binding protein
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
• UniProt Entry Name: PO5F1_HUMAN

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Uniprot Description

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of asymmetric cell division; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter