Rabbit anti-Human, Mouse OCT4 Polyclonal Antibody
Oct4 (POU5F1, OCT3, OCT4, OTF3, POU Domain, Class 5, Transcription Factor 1, Octamer-binding Protein 3, Octamer-binding Protein 4, Octamer-binding Transcription Factor 3)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
OCT4; Polyclonal Antibody; Oct4 (POU5F1; OCT3; OTF3; POU Domain; Class 5; Transcription Factor 1; Octamer-binding Protein 3; Octamer-binding Protein 4; Octamer-binding Transcription Factor 3); Anti -Oct4 (POU5F1; anti-OCT4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Oct4. Species Crossreactivity: mouse and primate.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Applicable Applications for anti-OCT4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Dilution: Western Blot: 1:1000-1:1500
ELISA: 1:350,000
Dilution: Western Blot: 1:1000-1:1500
ELISA: 1:350,000
Immunogen
Synthetic peptide corresponding to an internal region of human Oct4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-OCT4 antibody
Oct4, also known as POU domain transcription factor, Octomerbinding transcription factor 3 and Oct3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct4, which disappears rapidly when the cells differentiate. Most studies on Oct4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct4 expression; the protein appears in the nucleus, implying biological activity.
Functional Oct4 has been found in human amniotic fluid. As Oct4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct4 proteins within a cell. Oct4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.
Functional Oct4 has been found in human amniotic fluid. As Oct4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct4 proteins within a cell. Oct4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.
Product Categories/Family for anti-OCT4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Oct4
Similar Products
Product Notes
The OCT4 (Catalog #AAA6000914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Oct4 (POU5F1, OCT3, OCT4, OTF3, POU Domain, Class 5, Transcription Factor 1, Octamer-binding Protein 3, Octamer-binding Protein 4, Octamer-binding Transcription Factor 3) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OCT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Dilution: Western Blot: 1:1000-1:1500 ELISA: 1:350,000. Researchers should empirically determine the suitability of the OCT4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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