Western Blot (WB)
(Western blot analysis of OCT4 in A) uninduced and B) induced BL21 DE3 bacterial cells using MBS6000929 at 3ug/ml.)
Rabbit OCT4 Polyclonal Antibody
Oct4 (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC-9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein)
Reactivity
Bovine, Canine, Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
OCT4; Polyclonal Antibody; Oct4 (POU Domain; Class 5; Transcription Factor 1 Oct3/4; POU5F1; HGNC-9221; MGC22487; Octamer-binding Transcription Factor 3; Oct3; OTF3; OTF4; POU-type Homeodomain-containing DNA-binding Protein); Anti -Oct4 (POU Domain; anti-OCT4 antibody
Host
Rabbit
Reactivity
Bovine, Canine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human OCT4. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-OCT4 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 3-5ug/ml
Dilution: Western Blot: 3-5ug/ml
Immunogen
Synthetic peptide corresponding to aa230-244 (RKRKRTSIENRVRGN) of human OCT4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of OCT4 in A) uninduced and B) induced BL21 DE3 bacterial cells using MBS6000929 at 3ug/ml.)
Western Blot (WB)
(Western blot analysis of OCT4 in A) uninduced and B) induced BL21 DE3 bacterial cells using MBS6000929 at 3ug/ml.)
Related Product Information for anti-OCT4 antibody
OCT4 is a mammalian POU transcription factor expressed by early embryo cells and germ cells. The human OCT4 gene maps to chromosome 6 and has two isoforms with identical C-termini that include the characteristic POU-specific domain and the POU home domain. OCT4 is initially expressed in unfertilized oocytes and then in totipotent and pluripotent cells of the inner cell mass of pre-gastrulation embryos before becoming specific to germ-line cells. The OCT4 gene is expressed at low levels in such diverse adult tissues as heart, kidney, liver, spleen, and pancreatic islets. Specific functions of OCT4 have not been established. The expression of OCT4 in unfertilized oocytes and blastocysts is probably of greatest significance because of the role that OCT4 may be playing in early post fertilization events.
Product Categories/Family for anti-OCT4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Oct4
Similar Products
Product Notes
The OCT4 (Catalog #AAA6000929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Oct4 (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC-9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein) reacts with Bovine, Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OCT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 3-5ug/ml. Researchers should empirically determine the suitability of the OCT4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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