Rabbit OCT4 Polyclonal Antibody
Oct4 (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein)
Reactivity
Human, Rodent
Applications
ELISA
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
OCT4; Polyclonal Antibody; Oct4 (POU Domain; Class 5; Transcription Factor 1 Oct3/4; POU5F1; HGNC:9221; MGC22487; Octamer-binding Transcription Factor 3; Oct3; OTF3; OTF4; POU-type Homeodomain-containing DNA-binding Protein); Anti -Oct4 (POU Domain; anti-OCT4 antibody
Host
Rabbit
Reactivity
Human, Rodent
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Oct-4. Species Crossreactivity: rodent.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-OCT4 antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Dilution: ELISA (Serum Titer): 1:200,000
Dilution: ELISA (Serum Titer): 1:200,000
Immunogen
Synthetic peptide (16aa) aa282-297: RQKGKRSSSDYAQRED, coresponding to human Oct-4 (KLH). MW: of antigen: 38,.7kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-OCT4 antibody
Oct-4 (POU5F1) is a transcription factor that is expressed in undifferentiated embryonic stem cells and germ cells during normal development (1). A network of key factors that includes Oct-4, Nanog and Sox2, is necessary for the maintenance of pluripotent potential. Downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 has also been shown to be a useful marker for germ cell tumors (4). Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development. Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus, a critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. Data suggest that Oct3/4 is a master regulator of pluripotency that controls lineage commitment.
Product Categories/Family for anti-OCT4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Oct4
Similar Products
Product Notes
The OCT4 (Catalog #AAA617538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Oct4 (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein) reacts with Human, Rodent and may cross-react with other species as described in the data sheet. AAA Biotech's OCT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Dilution: ELISA (Serum Titer): 1:200,000. Researchers should empirically determine the suitability of the OCT4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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