Rabbit OCT4 Polyclonal Antibody | anti-OCT4 antibody

OCT4 Polyclonal Antibody

Cat. Num. AAA8527380

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: OCT4; Polyclonal Antibody; OCT4 Polyclonal Antibody; POU5F1; MGC22487; OCT3; OTF3; OTF4; anti-OCT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 265

• Applicable Applications for anti-OCT4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:200-1:500; IHC: 1:50-1:100; IF: 1:50-1:100; IP: 1:20-1:50
• Immunogen: Fusion protein of human OCT4
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using OCT4 antibody.)

Related Product Information for anti-OCT4 antibody

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2, 3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6, 7).

References

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50. 2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278. 3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. 4. Cheng, L. et al. (2007) J Pathol 211, 1-9. 5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20. 6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91. 7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

NCBI and Uniprot Product Information

• NCBI GI #: 553727233
• NCBI GeneID: 5460
• NCBI Accession #: NP_001272916.1
• NCBI GenBank Nucleotide #: NM_001285987.1
• UniProt Accession #: Q01860; P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
• Molecular Weight: 39kDa
• NCBI Official Full Name: POU domain, class 5, transcription factor 1 isoform 3
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Uniprot Description

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.